Reference : Selection and characterization of PCB-binding DNA aptamers.
Scientific journals : Article
Life sciences : Food science
http://hdl.handle.net/2268/122407
Selection and characterization of PCB-binding DNA aptamers.
English
Mehta, Jaytry [> > > >]
Rouah-Martin, Elsa [> > > >]
Van Dorst, Bieke [> > > >]
Maes, Bert [> > > >]
Herrebout, Wouter [> > > >]
Scippo, Marie-Louise mailto [Université de Liège - ULg > Département de sciences des denrées alimentaires > Analyse des denrées alimentaires]
Dardenne, Freddy [> > > >]
Blust, Ronny [> > > >]
Robbens, Johan [> > > >]
2012
Analytical Chemistry
American Chemical Society
84
3
1669-76
Yes (verified by ORBi)
International
0003-2700
1520-6882
Washington
DC
[en] Polychlorinated biphenyls (PCBs) are persistent organic pollutants (POPs) that resist natural degradation and bioaccumulate in nature. Combined with their toxicity, this leads them to cause cancer and other health hazards. Thus, there is a vital need for rapid and sensitive methods to detect PCB residues in food and in the environment. In this study, PCB-binding DNA aptamers were developed using PCB72 and PCB106 as targets for aptamer selection. Aptamers are synthetic DNA recognition elements which form unique conformations that enable them to bind specifically to their targets. Using in vitro selection techniques and fluorometry, an aptamer that binds with nanomolar affinity to both the PCBs has been developed. It displayed high selectivity to the original target congeners and limited affinity toward other PCB congeners (105, 118, 153, and 169), suggesting general specificity for the basic PCB skeleton with varying affinities for different congeners. This aptamer provides a basis for constructing an affordable, sensitive, and high-throughput assay for the detection of PCBs in food and environmental samples and offers a promising alternative to existing methods of PCB quantitation. This study therefore advances aptamer technology by targeting one of the highly sought-after POPs, for the first time ever recorded.
http://hdl.handle.net/2268/122407
10.1021/ac202960b
(c) 2011 American Chemical Society

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
Selection and characterization of PCB-binding DNA aptamers.pdfPublisher postprint1.42 MBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.