Reference : The Effect of Activating Fibroblast Growth Factor Receptor 3 Mutations on Osteogenic Dif...
Scientific journals : Article
Engineering, computing & technology : Multidisciplinary, general & others
http://hdl.handle.net/2268/122389
The Effect of Activating Fibroblast Growth Factor Receptor 3 Mutations on Osteogenic Differentiation and Ectopic Bone Formation by Human Periosteal Derived Cells
English
Bolander, Johanna [ > > ]
Roberts, Scott [ > > ]
Eyckmans, jeroen [ > > ]
Geris, Liesbet mailto [Université de Liège - ULg > Département d'aérospatiale et mécanique > Génie biomécanique >]
Luyten, Frank [ > > ]
2012
Journal of Tissue Science & Engineering
Omics Publishing Group
2
Yes (verified by ORBi)
International
2157-7552
[en] Activating mutations in Fibroblast Growth Factor Receptor 3 (FGFR3) have previously been shown to cause skeletal dysplasias through their effect on growth plate chondrocytes. However, the effect of FGFR3 mutations on bone progenitor cells may differ. The objective of this study was to investigate the effect of specific activating FGFR3 mutations on ectopic in vivo bone formation by periosteal derived cells (PDCs) seeded on calcium phosphate/ collagen scaffolds.
PDCs were isolated from hypochondroplasic (N540K mutation) and achondroplasic (G380R mutation) patients, along with age/sex matched controls. These cells were characterised in vitro for proliferation, osteogenic differentiation, FGFR3 signalling and in vivo bone formation. Subsequently, empirical modelling was used to find correlations between in vivo formed bone and in vitro cell behaviour. These data showed that in contrast to the G380R mutation, which produced no bone, the N540K mutation induced significant ectopic bone formation on specific carriers. This allowed correlation between percentage of induced bone formation to elevated in vitro proliferation and differentiation. Correlating osteogenic markers included Collagen type 1, alkaline phosphatase and osteocalcin. Enhanced proliferation was attributed to increased phosphorylation of Erk-1/2.
This study highlights the importance of FGFR3 in periosteal cell differentiation and also indicates it potential for targeted tissue engineering strategies.
Researchers
http://hdl.handle.net/2268/122389
10.4172/2157-7552.S2-003

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Open access
TisScEng_2012.pdfPublisher postprint1.69 MBView/Open

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.