Reference : Prolactin/growth hormone-derived antiangiogenic peptides highlight a potential role of t...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/12224
Prolactin/growth hormone-derived antiangiogenic peptides highlight a potential role of tilted peptides in angiogenesis
English
Nguyen, Ngoc-Quynh-Nhu mailto [Université de Liège - ULg > Département des sciences de la vie > Biologie et génétique moléculaire >]
Tabruyn, Sébastien mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Lins, Laurence mailto [Université de Liège - ULg > Chimie et bio-industries > Centre de Bio. Fond. - Section de Biologie moléc. et numér. >]
Lion, Michelle mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Cornet, Anne [Université de Liège - ULg > Département de morphologie et pathologie > Pathologie spéciale et autopsies >]
Lair, Florence [> > > >]
Rentier-Delrue, Françoise mailto [Université de Liège - ULg > Département des sciences de la vie > Biologie et génétique moléculaire >]
Brasseur, Robert mailto [Université de Liège - ULg > Chimie et bio-industries > Centre de Bio. Fond. - Section de Biologie moléc. et numér. >]
Martial, Joseph mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Struman, Ingrid mailto [Université de Liège - ULg > Département des sciences de la vie > Biologie et génétique moléculaire >]
2006
Proceedings of the National Academy of Sciences of the United States of America
National Academy of Sciences
103
39
14319-14324
Yes (verified by ORBi)
International
0027-8424
1091-6490
Washington
DC
[en] Alzheimer Disease
Amino Acid Sequence ; Amyloid beta-Protein/chemistry ; Angiogenesis Inhibitors/chemistry ; Animals ; Apoptosis/physiology ; Cattle ; Cell Proliferation ; Chick Embryo ; Endothelial Cells/cytology ; Gene Products, env/chemistry ; Growth Hormone/chemistry ; Humans ; Hydrophobicity ; Liposomes/metabolism ; Membrane Fusion/physiology ; Molecular Sequence Data ; Molecular Weight ; Mutant Proteins/metabolism ; Neovascularization, Physiologic/physiology ; Peptide Fragments/chemistry ; Prolactin/chemistry ; Rats ; Recombinant Proteins/biosynthesis ; Retroviridae Proteins, Oncogenic/chemistry ; Viral Fusion Proteins/chemistry
[en] Angiogenesis is a crucial step in many pathologies, including tumor growth and metastasis. Here, we show that tilted peptides exert antiangiogenic activity. Tilted (or oblique-oriented) peptides are short peptides known to destabilize membranes and lipid cores and characterized by an asymmetric distribution of hydrophobic residues along the axis when helical. We have previously shown that 16-kDa fragments of the human prolactin/growth hormone (PRL/GH) family members are potent angiogenesis inhibitors. Here, we demonstrate that all these fragments possess a 14-aa sequence having the characteristics of a tilted peptide. The tilted peptides of human prolactin and human growth hormone induce endothelial cell apoptosis, inhibit endothelial cell proliferation, and inhibit capillary formation both in vitro and in vivo. These antiangiogenic effects are abolished when the peptides' hydrophobicity gradient is altered by mutation. We further demonstrate that the well known tilted peptides of simian immunodeficiency virus gp32 and Alzheimer's beta-amyloid peptide are also angiogenesis inhibitors. Taken together, these results point to a potential new role for tilted peptides in regulating angiogenesis.
Researchers ; Professionals
http://hdl.handle.net/2268/12224
also: http://hdl.handle.net/2268/63355
10.1073/pnas.0606638103

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