Article (Scientific journals)
Leukemic cell xenograft in zebrafish embryo for investigating drug efficacy.
Pruvot, Benoist; Jacquel, Arnaud; Droin, Nathalie et al.
2011In Haematologica, 96 (4), p. 612-6
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Keywords :
Animals; Antineoplastic Agents/pharmacology; Cell Line, Tumor/drug effects; Dose-Response Relationship, Drug; Drug Evaluation, Preclinical; Humans; Jurkat Cells; K562 Cells; Piperazines/pharmacology; Pyrimidines/pharmacology; Tretinoin/pharmacology; Xenograft Model Antitumor Assays; Zebrafish/surgery
Abstract :
[en] Zebrafish were proposed as an alternative to mammalian models to assess the efficacy and toxicity of antileukemic drugs. Due to the limited number of transgenic zebrafish leukemia models, we explored human leukemic cell xenograft in zebrafish embryos. Human leukemic cell lines and blast cells sorted from patients with acute myelogenous leukemia were injected 48 hours post-fertilization and remained in the circulation of zebrafish embryos for several days without affecting their development. Imatinib and oxaphorines did not demonstrate any toxicity on normal zebrafish embryos and decreased the leukemic burden in animals xenografted with sensitive leukemic cell lines. Two other molecules, all-trans retinoic acid and the translation inhibitor 4EGI-1, demonstrated teratogenic effects at concentrations shown to be efficient in vitro, which precluded investigation of their antileukemic activity in such models. Altogether, xenografted leukemic cells in zebrafish embryos are a pharmacologically relevant model for screening non-teratogenic drugs.
Research center :
AFFISH-RC - Applied and Fundamental FISH Research Center - ULiège
Giga-Development and Stem Cells - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Pruvot, Benoist ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Jacquel, Arnaud
Droin, Nathalie
Auberger, Patrick
Bouscary, Didier
Tamburini, Jerome
Muller, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie et génétique moléculaire
Fontenay, Michaela
Chluba, Johanna
Solary, Eric
Language :
English
Title :
Leukemic cell xenograft in zebrafish embryo for investigating drug efficacy.
Publication date :
2011
Journal title :
Haematologica
ISSN :
0390-6078
eISSN :
1592-8721
Publisher :
Ferrata Storti Foundation, Pavia, Italy
Volume :
96
Issue :
4
Pages :
612-6
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 16 May 2012

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