Reference : Leukemic cell xenograft in zebrafish embryo for investigating drug efficacy.
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Leukemic cell xenograft in zebrafish embryo for investigating drug efficacy.
Pruvot, Benoist [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire]
Jacquel, Arnaud [> > > >]
Droin, Nathalie [> > > >]
Auberger, Patrick [> > > >]
Bouscary, Didier [> > > >]
Tamburini, Jerome [> > > >]
Muller, Marc mailto [Université de Liège - ULg > Département des sciences de la vie > Biologie et génétique moléculaire >]
Fontenay, Michaela [> > > >]
Chluba, Johanna [> > > >]
Solary, Eric [> > > >]
Ferrata Storti Foundation
Yes (verified by ORBi)
[en] Animals ; Antineoplastic Agents/pharmacology ; Cell Line, Tumor/drug effects ; Dose-Response Relationship, Drug ; Drug Evaluation, Preclinical ; Humans ; Jurkat Cells ; K562 Cells ; Piperazines/pharmacology ; Pyrimidines/pharmacology ; Tretinoin/pharmacology ; Xenograft Model Antitumor Assays ; Zebrafish/surgery
[en] Zebrafish were proposed as an alternative to mammalian models to assess the efficacy and toxicity of antileukemic drugs. Due to the limited number of transgenic zebrafish leukemia models, we explored human leukemic cell xenograft in zebrafish embryos. Human leukemic cell lines and blast cells sorted from patients with acute myelogenous leukemia were injected 48 hours post-fertilization and remained in the circulation of zebrafish embryos for several days without affecting their development. Imatinib and oxaphorines did not demonstrate any toxicity on normal zebrafish embryos and decreased the leukemic burden in animals xenografted with sensitive leukemic cell lines. Two other molecules, all-trans retinoic acid and the translation inhibitor 4EGI-1, demonstrated teratogenic effects at concentrations shown to be efficient in vitro, which precluded investigation of their antileukemic activity in such models. Altogether, xenografted leukemic cells in zebrafish embryos are a pharmacologically relevant model for screening non-teratogenic drugs.
Applied and Fundamental FISH Research Center - AFFISH-RC ; Giga-Development and Stem Cells
"Obtained from Haematologica/the Hematology Journal website"

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