Contents The effect of cysteamine on in vitro nuclear and cytoplasmic maturation of equine oocytes collected by transvaginal ultrasound guided follicular aspiration was assessed.pdf
[en] Contents The effect of cysteamine on in vitro nuclear and cytoplasmic maturation of equine oocytes collected by transvaginal ultrasound guided follicular aspiration was assessed. Oocytes were matured in vitro with (cysteamine group) or without (control group) cysteamine. The nuclear stage after DNA Hoechst staining, penetration rates after two different in vitro fertilization (IVF) techniques (IVF media with ionophore and Hepes buffer with heparin) and the embryo yield following oocyte intra-oviductal transfer were used as a criterion for assessing nuclear and cytoplasmic maturation, respectively. Contrary to the data described in other domestic species, there was no effect of cysteamine on in vitro nuclear maturation, IVF or in vivo embryonic development under our conditions. Ovum pick up yields (52%) and maturation rates (control group: 47% and cysteamine group: 55%) were similar to those previously reported. From 57 oocytes transferred to the oviduct in each group, the number of embryos collected was 10 (17%) in the control group and five in the cysteamine group (9%). Those two percentages were not statistically different (p > 0.05). No effect of IVF technique was seen on the success rate (6%) in each group.
Disciplines :
Veterinary medicine & animal health Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Deleuze, Stefan ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux - Département clinique des animaux de compagnie et des équidés
Dubois, Clotilde
Caillaud, Maud
Bruneau, Bernard
Goudet, Ghylène
Duchamp, Guy
Language :
English
Title :
Influence of cysteamine on in vitro maturation, in vitro and in vivo fertilization of equine oocytes
Alm H, Torner H, Blottner S, Nurnberg G, Kanitz W, 2001: Effect of sperm cryopreservation and treatment with calcium ionophore or heparin on in vitro fertilization of horse oocytes. Theriogenology 56, 817-829.
Alvarenga MA, McCue PM, Bruemmer J, Neves Neto JR, Squires EL, 2001: Ovarian superstimulatory response and embryo production in mares treated with equine pituitary abstract twice daily. Theriogenology 56, 879-887.
Anand T, Kumar D, Chauhan MS, Manik RS, Palta P, 2008: Cysteamine supplementation of in vitro maturation medium, in vitro culture medium or both media promotes in vitro development of buffalo (Bubalus bubalis) embryos. Reprod Fertil Dev 20, 253-257.
Bezard J, Magistrini M, Battut I, Duchamp G, Palmer E, 1992: Fecondation in vitro chez les équidés. Rec Med Vet 168, 993-1003.
Bezard J, Goudet G, Duchamp G, Palmer E, 1995: Preovulatory maturation of ovarian follicles and oocytes in unstimulated and superovulatory mares. Biol Reprod Mono 1, 261-271.
Bing YZ, Naga T, Rodriguez-Martinez H, 2001: Effects of cysteamine, fsh and estradiol-17beta on in vitro maturation of porcine oocytes. Theriogenology 55, 867-876.
Bing YZ, Hirao Y, Iga K, Che LM, Takenouchi N, Kuwayama M, Fuchimoto D, Rodriguez-Martinez H, Nagai T, 2002: In vitro maturation and glutathione synthesis of porcine oocytes in the presence or absence of cysteamine under different oxygen tensions: role of cumulus cells. Reprod Fertil Dev 14, 125-131.
Bogh IB, Bezard J, Duchamp G, Baltsen M, Gerard N, Daels P, Greve T, 2002: Pure pre-ovulatory follicular fluid promotes in vitro maturation of in vivo aspirated equine oocytes. Theriogenology 57, 1765-1779.
Bruck I, Raun K, Synnestvedt B, Greve T, 1992: Follicle aspiration in the mare using a transvaginal ultrasoundguided technique. Equine Vet J 24, 58-59.
Carneiro GF, Liu IK, Hyde D, Andertson GB, Lorenzo PL, Ball BA, 2002: Quantification and distribution of equine oocyte cortical granules during meiotic maturation and after activation. Mol Reprod Dev 63, 451-458.
Carnevale EM, 2004: Oocyte transfer and gamete intrafallopian transfer in the mare. Anim Reprod Sci 82-83, 617-624.
Carnevale EM, Ginther OJ, 1995: Defective oocytes as a cause of subfertility in old mares. Biol Reprod Mono 1, 209-214.
Carnevale EM, Coutinho da Silva MA, Panzani D, Stokes JE, Squires EL, 2005: Factors affecting the success of oocyte transfer in a clinical program for subfertile mares. Theriogenology 64, 519-527.
Choi YH, Okada Y, Hochi S, Braun J, Sato K, Oguri N, 1994: In vitro fertilization rate of horse oocytes with partially removed zonae. Theriogenology 42, 795-802.
Choi YH, Love CC, Love LB, Varner DD, Brinsko S, Hinrichs K, 2002: Developmental competence in vivo and in vitro of in vitro-matured equine oocytes fertilized by intracytoplasmic sperm injection with fresh or frozenthawed spermatozoa. Reproduction 123, 455-465.
Choi YH, Love CC, Varner DD, Hinrichs K, 2006a: Equine blastocyst development after intracytoplasmic injection of sperm subjected to two freeze-thaw cycles. Theriogenology 65, 808-819.
Choi YH, Love LB, Varner DD, Hinrichs K, 2006b: Holding immature equine oocytes in the absence of meiotic inhibitors: Effect on germinal vesicle chromatin and blastocyst development after intracytoplasmic sperm injection. Theriogenology 66, 955-963.
Daels P, Duchamp G, Goudet G, 2001: Oocyte transfer: pregnancy rates using oocytes collected at 24 and 36 hours after induction of ovulation. In: Stont TAE and Wade JF (ed.), Second Meeting of the European Equine Gamete Group (EEGG), Vol. 5. Loosdrecht, The Netherlands, pp. 25.
Dell'Aquila ME, Fusco S, Lacalandra GM, Maritato F, 1996: In vitro maturation and fertilization of equine oocytes recovered during the breeding season. Theriogenology 45, 547-560.
Dell'Aquila ME, Cho YS, Minoia P, Traina V, Fusco S, Lacalandra GM, Maritato F, 1997a: Intracytoplasmic sperm injection (ICSI) versus conventional IVF on abattoir-derived and in vitro-matured equine oocytes. Theriogenology 47, 1139-1156.
Dell'Aquila ME, Cho YS, Minoia P, Traina V, Lacalandra GM, Maritato F, 1997b: Effects of follicular fluid supplementation of in vitro maturation medium on the fertilization and development of equine oocytes after in-vitro fertilization or intracytoplasmic sperm injection. Hum Reprod 12, 2766-2772.
Dippert KD, Squires EL, 1994: Fertilization rates in superovulated and spontaneously ovulating mares. Theriogenology 41, 1411-1423.
Duchamp G, Bour B, Combarnous Y, Palmer E, 1987: Alternative solutions to hCG induction of ovulation in the mare. J Reprod Fertil Suppl 35, 221-228.
Duchamp G, Bezard J, Palmer E, 1994: Oocyte yield and the consequences of puncture of all follicles larger than 8 millimeters in mares. In: Sharp DC, Bazer FW (eds), 6th International Symposium on Equine Reproduct. Society for the Study of Reproduction, Ann Arbor (USA), Caxambu (BRA), pp. 233-241.
Galli C, Crotti G, Duchi R, Mari G, Zagaglia G, Duchamp G, Daels P, Lazzari G, 2002: Frozen-thawed embryos produced by ovum pick up of immature oocytes and ICSI are capable to establish pregnancies in the horse. Theriogenology 58, 705-708.
Gasparrini B, Neglia G, Palo RD, Campanile G, Zicarelli L, 2000: Effect of cysteamine during in vitro maturation on buffalo embryo development. Theriogenology 54, 1537-1542.
Gasparrini B, Sayoud H, Neglia G, de Matos DG, Donnay I, Zicarelli L, 2003: Glutathione synthesis during in vitro maturation of buffalo (Bubalus bubalis) oocytes: effects of cysteamine on embryo development. Theriogenology 60, 943-952.
Goudet G, Bezard J, Duchamp G, Gerard N, Palmer E, 1997a: Equine oocyte competence for nuclear and cytoplasmic in vitro maturation: effect of follicle size and hormonal environment. Biol Reprod 57, 232-245.
Goudet G, Bezard J, Duchamp G, Palmer E1997b: Transfer of immature oocytes to a pre-ovulatory follicle: an alternative to in vitro maturation in the mare? Equine Vet J 25(Suppl), 54-59.
Goudet G, Belin F, Bezard J, Gerard N, 1998a: Maturationpromoting factor (MPF) and mitogen activated protein kinase (MAPK) expression in relation to oocyte competence for in vitro maturation in the mare. Mol Hum Reprod 4, 563-570.
Goudet G, Bezard J, Belin F, Duchamp G, Palmer E, Gerard N, 1998b: Oocyte competence for in vitro maturation is associated with histone H1 kinase activity and is influenced by estrous cycle stage in the mare. Biol Reprod 59, 456-462.
Goudet G, Belin F, Mlodawska W, Bezard J, 2000: Influence of epidermal growth factor on in vitro maturation of equine oocytes. J Reprod Fertil Suppl 56, 482-492.
Grondahl C, Hyttel P, Grondahl ML, Eriksen T, Gotfredsen P, Greve T, 1995: Structural and endocrine aspects of equine oocyte maturation in vivo. Mol Reprod Dev 42, 94-105.
Grupen CG, Nagashima H, Nottle MB, 1995: Cysteamine enhances in vitro development of porcine oocytes matured and fertilized in vitro. Biol Reprod 53, 173-178.
Guerand M, Mahla R, Lagneaux D, Amigues Y, Palmer E, Bezard J1997: Parentage testing of Day-10 equine embryos by amplified PCR analysis of microsatellites Equine Vet J 25(Suppl), 69-71
Hinrichs K, Digiorgio LM, 1991: Embryonic development after intra-follicular transfer of horse oocytes. J Reprod Fertil Suppl 44, 369-374.
Hinrichs K, Matthews GL, Freeman DA1997: Effect of oocyte maturation time on embryo development after oocyte transfer in the mare. Theriogenology 47, 392.
Hinrichs K, Love CC, Brinsko SP, Choi YH, Varner DD, 2002: In vitro fertilization of in vitro-matured equine oocytes: effect of maturation medium, duration of maturation, and sperm calcium ionophore treatment, and comparison with rates of fertilization in vivo after oviductal transfer. Biol Reprod 67, 256-262.
Hinrichs K, Choi YH, Love LB, Varner DD, Love CC, Walckenaer BE, 2005: Chromatin configuration within the germinal vesicle of horse oocytes: changes post-mortem and relationship to meiotic and developmental competence. Biol Reprod 72, 1142-1150.
Hossein MS, Kim MK, Jang G, Oh HJ, Koo O, Kim JJ, Kang SK, Lee BC, Hwang WS, 2007: Effects of thiol compounds on in vitro maturation of canine oocytes collected from different reproductive stages. Mol Reprod Dev 74, 1213-1220.
Luciano AM, Goudet G, Perazzoli F, Lahuec C, Gerard N, 2006: Glutathione content and glutathione peroxidase expression in in vivo and in vitro matured equine oocytes. Mol Reprod Dev 73, 658-666.
Luderer U, Kavanagh TJ, White CC, Faustman EM, 2001: Gonadotropin regulation of glutathione synthesis in the rat ovary. Reprod Toxicol, 15, 495-504.
de Matos DG, Furnus CC, 2000: The importance of having high glutathione (GSH) level after bovine in vitro maturation on embryo development effect of beta-mercaptoethanol, cysteine and cystine. Theriogenology 53, 761-771.
de Matos DG, Furnus CC, Moses DF, Baldassarre H, 1995: Effect of cysteamine on glutathione level and developmental capacity of bovine oocyte matured in vitro. Mol Reprod Dev 42, 432-436.
de Matos DG, Furnus CC, Moses DF, Martinez AG, Matkovic M, 1996: Stimulation of glutathione synthesis of in vitro matured bovine oocytes and its effect on embryo development and freezability. Mol Reprod Dev 45, 451-457.
de Matos DG, Gasparrini B, Furnus CC, Thomos JG, 1999: Glutathione synthesis during in vitro maturation of ovine oocytes: Effects of cysteamine and b-mercaptoethanol. Theriogenology 50, 368.
de Matos DG, Gasparrini B, Pasqualini SR, Thompson JG, 2002: Effect of glutathione synthesis stimulation during in vitro maturation of ovine oocytes on embryo development and intracellular peroxide content. Theriogenology 57, 1443-1451.
McKinnon AO, Carnevale EM, Squires EL, 1988: Heterogenous and xenogenous fertilization of equine oocytes. J Equine Vet Sci 8, 143-147.
Neumann H, Alm H, Schnurrbusch U, Torner H, Greising T, Kanitz W, 1995: The ultrastructure of equine oocytes before and after in vitro maturation. Reprod Domest Anim 30, 428.
Palmer E, Bezard J, Magistrini M, Duchamp G, 1991: In vitro fertilization in the horse. a retrospective study. J Reprod Fertil 44(Suppl), 375-384.
Perreault SD, Barbee RR, Slott VL, 1988: Importance of glutathione in the acquisition and maintenance of sperm nuclear decondensing activity in maturing hamster oocytes. Dev Biol 125, 181-186.
Ray BS, Squires EL, Cook NL, 1994: Pregnancy following gamete intrafallopian transfer in the mare. J Equine Vet Sci 14, 27-30.
Scott TJ, Carnevale EM, Maclellan LJ, Scoggin CF, Squires EL, 2001: Embryo development rates after transfer of oocytes matured in vivo, in vitro, or within oviducts of mares. Theriogenology 55, 705-715.
Shabpareh V, Squires EL, Seidel GE, Jasko DJ, 1993: Methods for collecting and maturing equine oocytes in vitro. Theriogenology 40, 1161-1175.
Squires EL, 1996: Maturation and fertilization of equine oocytes. Vet Clin North Am Equine Pract 12, 31-45.
Takahashi M, Nagai T, Hamano S, Kuwayama M, Okamura N, Okano A, 1993: Effect of thiol compounds on in vitro development and intracellular glutathione content of bovine embryos. Biol Reprod 49, 228-232.
Tremoleda JL, Schoevers EJ, Stout TA, Colenbrander B, Bevers MM, 2001: Organisation of the cytoskeleton during in vitro maturation of horse oocytes. Mol Reprod Dev 60, 260-269.
Whitaker BD, Knight JW, 2004: Exogenous gamma-glutamyl cycle compounds supplemented to in vitro maturation medium influence in vitro fertilization, culture, and viability parameters of porcine oocytes and embryos. Theriogenology 62, 311-322.
Yamauchi N, Nagai T, 1999: Male pronuclear formation in denuded porcine oocytes after in vitro maturation in the presence of cysteamine. Biol Reprod 61, 828-833.
Yoshida M, Ishigaki K, Nagai T, Chikyu M, Pursel VG, 1993: Glutathione concentration during maturation and after fertilization in pig oocytes: relevance to the ability of oocytes to form male pronucleus. Biol Reprod 49, 89-94.
Zhang JJ, Boyle MS, Allen WR, 1989: Recent studies on in vitro fertilization of equine oocytes. Equine Vet J 21, 101-104.
Zhang JJ, Muzs LZ, Boyle MS, 1990: In vitro fertilization of horse follicular oocytes matured in vitro. Mol Reprod Dev 26, 361-365.
