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Article (Scientific journals)
Stabilization of T7-Promoter-Based Parhs Expression Vectors Using the Parb Locus
De Moerlooze, L.; Struman, Ingrid; Renard, Andre et al.
1992In Gene, 119 (1), p. 91-3
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Abstract :
[en] We describe a modification of the pAR3040 vector which results in its efficient stabilization during cell division. The parB locus of the plasmid R1 was introduced into the plasmid, pAR3040, to construct the pARHS vectors. These vectors are stable for at least 60 cell generations, even in the absence of selection by an antibiotic present in the culture media, both with or without IPTG induction.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
De Moerlooze, L.
Struman, Ingrid  ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie et génétique moléculaire
Renard, Andre
Martial, Joseph ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Language :
English
Title :
Stabilization of T7-Promoter-Based Parhs Expression Vectors Using the Parb Locus
Publication date :
21 September 1992
Journal title :
Gene
ISSN :
0378-1119
eISSN :
1879-0038
Publisher :
Elsevier, Netherlands
Volume :
119
Issue :
1
Pages :
91-3
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 06 May 2009

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