Article (Scientific journals)
Differential expression of c-fos, Hsp70 and Hsp27 after photothrombotic injury in the rat brain.
Plumier, Jean-Christophe; Armstrong, J. N.; Wood, N. I. et al.
1997In Molecular Brain Research, 45 (2), p. 239-46
Peer Reviewed verified by ORBi
 

Files


Full Text
Mol Brain Res 1997.pdf
Publisher postprint (542.37 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Animals; Brain Injuries/metabolism/pathology; Cerebral Cortex/metabolism/pathology; Fiber Optic Technology; Frontal Lobe/injuries; Gene Expression; HSP70 Heat-Shock Proteins/biosynthesis; Heat-Shock Proteins/biosynthesis; Intracranial Embolism and Thrombosis/metabolism/pathology; Male; Necrosis; Parietal Lobe/injuries; Protein Biosynthesis; Proto-Oncogene Proteins c-fos/biosynthesis; RNA, Messenger/biosynthesis; Rats; Rats, Inbred Strains; Transcription, Genetic
Abstract :
[en] In situ hybridization and immunohistochemistry were used to examine the expression of c-fos, Hsp70 and Hsp27 following photothrombotic injury in the right fronto-parietal cortex of the rat. C-fos mRNA and protein were detected in the entire cerebral cortex on the lesioned side. Hsp70 mRNA accumulation was observed only adjacent and peripheral to the site of the lesion. At 1 h after photothrombotic injury, Hsp70 expression delineates the area of necrosis at 24 h after photothrombotic injury. Hsp27 protein was observed in the ipsilateral cerebral cortex with the exception of the deep layers of the cingulate cortex. In addition, while c-Fos immunoreactivity was localized in cell nuclei, Hsp27 immunoreactivity was detected in the cytoplasm of astrocytes. These results demonstrate that unilateral cortical injury induces changes in gene expression that vary according to cell type and brain region.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Plumier, Jean-Christophe ;  Dalhousie University > Anatomy and Neurobiology
Armstrong, J. N.;  Dalhousie University > Pharmacology, Anatomy and Neurobiology
Wood, N. I.;  SmithKline Beecham Pharmaceuticals
Babity, J. M.;  Dalhousie University > Pharmacology, Anatomy and Neurobiology
Hamilton, T. C.;  SmithKline Beecham Pharmaceuticals
Hunter, A. J.;  SmithKline Beecham Pharmaceuticals
Robertson, H. A.;  Dalhousie University > Pharmacology
Currie, R. W.;  Dalhousie University > Anatomy and Neurobiology
Language :
English
Title :
Differential expression of c-fos, Hsp70 and Hsp27 after photothrombotic injury in the rat brain.
Publication date :
1997
Journal title :
Molecular Brain Research
ISSN :
0169-328X
Publisher :
Elsevier Science
Volume :
45
Issue :
2
Pages :
239-46
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 06 May 2009

Statistics


Number of views
43 (4 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
38
Scopus citations®
without self-citations
31
OpenCitations
 
36

Bibliography


Similar publications



Contact ORBi