Article (Scientific journals)
A novel seizure-induced synaptotagmin gene identified by differential display.
Babity, J. M.; Armstrong, J. N.; Plumier, Jean-Christophe et al.
1997In Proceedings of the National Academy of Sciences of the United States of America, 94 (6), p. 2638-41
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Keywords :
Amino Acid Sequence; Animals; Base Sequence; Blotting, Northern; Brain/metabolism; Calcium-Binding Proteins; Epilepsy, Temporal Lobe/metabolism; Hippocampus/metabolism; Humans; Kainic Acid; Male; Membrane Glycoproteins/biosynthesis/genetics; Molecular Sequence Data; Nerve Tissue Proteins/biosynthesis/genetics; Parietal Lobe/metabolism; RNA, Messenger/biosynthesis; Rats; Rats, Sprague-Dawley; Seizures/chemically induced/metabolism; Sequence Homology, Amino Acid; Skates (Fish); Synaptotagmins; Transcription, Genetic
Abstract :
[en] Systemic administration of kainic acid, a cyclic analogue of glutamate, produces many of the clinical features of human temporal lobe epilepsy and status epilepticus in rats, including the induction of motor convulsions and the degeneration of neurons in the hippocampus and piriform cortex. Differential display PCR was used to identify mRNAs that are differentially expressed between degenerating and nondegenerating tissues in the brain after kainic acid-induced seizure activity. A novel cDNA fragment expressed in the degenerating hippocampus and piriform cortex, but not in the nondegenerating parietal cortex, was identified, cloned, and sequenced. This novel cDNA fragment identified a new member of the synaptotagmin gene family that is rapidly and transiently induced in response to seizure activity. Differential expression of this synaptotagmin gene, syt X, was confirmed by Northern blot analysis and in situ hybridization. This novel, inducible synaptotagmin gene may provide a direct link between seizure-induced neuronal gene expression and subsequent modulation of synaptic structure and function.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Biochemistry, biophysics & molecular biology
Author, co-author :
Babity, J. M.;  Dalhousie University > Pharmacology, Anatomy and Neurobiology
Armstrong, J. N.;  Dalhousie University > Pharmacology, Anatomy and Neurobiology
Plumier, Jean-Christophe ;  Dalhousie University > Anatomy and Neurobiology
Currie, R. W.;  Dalhousie University > Anatomy and Neurobiology
Robertson, H. A.;  Dalhousie University > Pharmacology
Language :
English
Title :
A novel seizure-induced synaptotagmin gene identified by differential display.
Publication date :
1997
Journal title :
Proceedings of the National Academy of Sciences of the United States of America
ISSN :
0027-8424
eISSN :
1091-6490
Publisher :
National Academy of Sciences, Washington, United States - District of Columbia
Volume :
94
Issue :
6
Pages :
2638-41
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 06 May 2009

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