Reference : Genital re-excretion of Murid gammaherpesvirus 4 following intranasal infection

	Document type :	Scientific congresses and symposiums : Paper published in a book
	Discipline(s) :	Life sciences : Microbiology
	To cite this reference:	http://hdl.handle.net/2268/121615

Title :	Genital re-excretion of Murid gammaherpesvirus 4 following intranasal infection
Language :	English
Author, co-author :	François, Sylvie [Université de Liège - ULg > > Immunologie et vaccinologie >]
	Vidick, Sarah [Université de Liège - ULg > > Immunologie et vaccinologie >]
	Sarlet, Michaël [Université de Liège - ULg > Département de morphologie et pathologie > Département de morphologie et pathologie >]
	Desmecht, Daniel [Université de Liège - ULg > Département de morphologie et pathologie > Pathologie spéciale et autopsies >]
	Stevenson, Philip G. [University of Cambridge > Division of Virology > Department of Pathology > >]
	Drion, Pierre [Université de Liège - ULg > Département des sciences biomédicales et précliniques > GIGA-R:Méth. expér.des anim. de labo et éth. en expér. anim. >]
	Vanderplasschen, Alain [Université de Liège - ULg > > Immunologie et vaccinologie >]
	Gillet, Laurent [Université de Liège - ULg > > Immunologie et vaccinologie >]
Publication date :	9-Dec-2011
Main document title :	Proceedings of the 1st Scientific Meeting of the Faculty of Veterinary Medicine
ISBN :	9782875430052
Event name :	1st Scientific Meeting of the Faculty of Veterinary Medicine
Event date :	09 décembre 2011
Event place (city) :	Liège
Event country :	Belgium
Abstract :	[en] Gammaherpesviruses are the archetypes of persistent viruses that have been identified in a range of animals from mice to man. As the human gammaviruses have no well-established in vivo infection model, related animal gammaherpesviruses are an important source of information. We are studying Murid herpesvirus 4 (MuHV-4) in inbred laboratory mouse strains which are commonly accepted as a good model for studying gammaherpesviruses in vivo. To date, it has however never been possible to monitor viral reexcretion and virus transmission in this species. In order to identify potential re-excretion sites, intranasally infected mice were followed through global luciferase imaging for up to six months after infection. Surprisingly, we detected transient viral replication in mice genital tract at various times after latency establishment. Ex vivo imaging, quantitative PCR and immunohistochemistry revealed that virus genomes were present in high quantity in the vaginal tissue and that viral replication occurred mainly at the vaginal external border. Moreover, we highlighted the presence of free infectious viruses in the vaginal cavity at the moment of the observation of viral replication. As this ephemeral viral reexcretion could reveal a link with reproductive cycle, we compared reexcretion in normal and ovariectomized mice. Interestingly, no viral reactivation was observed in absence of hormonal cycle. In conclusion, we experimentally indentified for the first time a reexcretion site for MuHV-4 in mice that had been intranasaly infected. In the future, these results could help us to better understand the biology of gammaherpesviruses but should also allow us to develop strategies that could prevent the spread of these viruses in natural populations.
Permalink :	http://hdl.handle.net/2268/121615

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