Reference : Evaluation of plasma carboxy-terminal cross-linking telopeptide of type I collagen conce...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/121307
Evaluation of plasma carboxy-terminal cross-linking telopeptide of type I collagen concentration in horses.
English
Carstanjen, Bianca [> > > >]
Hoyle, Nicholas R [> > > >]
Gabriel, Annick mailto [Université de Liège - ULg > Département de morphologie et pathologie > Anatomie des animaux domestiques]
Hars, Olaf [> > > >]
Sandersen, Charlotte mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Département clinique des animaux de compagnie et des équidés]
Amory, Hélène mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Médecine interne des équidés]
Remy, Benoît mailto [Université de Liège - ULg > > GIGA-Management : Plate-forme transgénique]
2004
American Journal of Veterinary Research
American Veterinary Medical Association
65
1
104-9
Yes (verified by ORBi)
International
0002-9645
Schaumburg
IL
[en] Age Factors ; Animals ; Chemiluminescent Measurements ; Circadian Rhythm/physiology ; Collagen/blood ; Collagen Type I ; Horses/blood/physiology ; Immunosorbent Techniques ; Osteocalcin/blood ; Peptides/blood ; Radioimmunoassay
[en] OBJECTIVE: To evaluate a human assay for quantification of carboxy-terminal cross-linking telopeptide of type I collagen (CTX-I), assess the influence of age on plasma CTX-I concentration, investigate the relationship between plasma CTX-I and serum osteocalcin concentrations, and determine whether concentrations of plasma CTX-I or serum osteocalcin fluctuate in circadian manner in horses. HORSES: 75 clinically normal horses. PROCEDURE: Cross-reactivity between equine serum CTX-I and CTX-I antibodies in an automated electrochemiluminescent sandwich antibody assay (ECLIA) was evaluated via a specificity test (ie, dilution test) and recovery calculation. Serum osteocalcin concentration was measured with an equine-specific osteocalcin radioimmunoassay. To analyze diurnal variations in plasma CTX-I and serum osteocalcin concentrations, blood samples were obtained hourly during a 24-hour period. RESULTS: Results of the dilution test indicated good correlation (r > 0.99) between expected serum CTX-I concentrations and measured serum CTX-I concentrations. The calculated CTX-I recovery was 97.6% to 109.9%. Plasma CTX-I and serum osteocalcin concentrations were correlated. Plasma CTX-I concentration was inversely correlated with age of the horse. No significant circadian variations in plasma CTX-I and serum osteocalcin concentrations were detected. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that the fully automated CTX-I ECLIA can be used for evaluation of plasma and serum samples from horses and may be a useful tool to monitor bone metabolism changes. Horses in this study did not have notable diurnal fluctuations in serum osteocalcin and plasma CTX-I concentrations.
Researchers ; Professionals
http://hdl.handle.net/2268/121307

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
Carstanjen et al_AJVR_2004.pdfPublisher postprint442.52 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.