Reference : Cytokine production from sputum cells after allergenic challenge in IgE-mediated asthma
Scientific journals : Article
Human health sciences : Cardiovascular & respiratory systems
http://hdl.handle.net/2268/12022
[en] Cytokine production from sputum cells after allergenic challenge in IgE-mediated asthma
English
Bettiol, Jeanne [> > > >]
Sele, Jocelyne [Centre Hospitalier Universitaire de Liège - CHU > > Pneumologie-Allergologie >]
Henket, Monique [Centre Hospitalier Universitaire de Liège - CHU > > Pneumologie-Allergologie >]
Louis, Edouard [Université de Liège - ULg > Département des sciences cliniques > Hépato-gastroentérologie >]
Malaise, Michel [Université de Liège - ULg > Département des sciences cliniques > Rhumatologie]
Bartsch, Pierre [Université de Liège - ULg > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine)]
Louis, Renaud mailto [Université de Liège - ULg > Département des sciences cliniques > Pneumologie - Allergologie]
Dec-2002
Allergy
Blackwell Publishing
57
12
1145-1150
Yes (verified by ORBi)
International
0105-4538
1398-9995
Oxford
United Kingdom
[en] allergen ; asthma ; cell culture ; cytokines ; sputum ; Cytokines/biosynthesis/drug effects ; Eosinophils/drug effects/metabolism ; Epithelial Cells/drug effects/metabolism ; Female ; Humans ; Hypersensitivity, Immediate/etiology/metabolism/physiopathology ; Leukocyte Count ; Lymphocytes/drug effects/metabolism ; Macrophages/drug effects/metabolism ; Male ; Middle Aged ; Neutrophils/drug effects/metabolism ; Sodium Chloride/administration & dosage/adverse effects ; Sputum/cytology/metabolism
Time Factors
[en] Background: Th2 cytokine production from airway cells is thought to govern the eosinophilic airways in ammation in allergic asthma. Induced sputum has become a widely used technique to assess airways in ammation. Methods: By applying the technique of induced sputum to collect airways cells, we have assessed the spontaneous production of a set of cytokines, including interleukin-4, 6, 10, interferon-gamma and tumour necrosis factor-alpha 6 h after a bronchial allergenic hallenge with Dermatophagoides pteronyssinus (Dpt) in 12 sensitized asthmatics and compared the results obtained after inhalation of saline as control. A group of eight healthy non-allergic subjects was enrolled to control for any non-specific effect of Dpt. Cytokines were measured by a dynamic immunoassay during a 24-h sputum cell culture. Results: Allergen challenge in sensitized asthmatics caused an acute and a late bronchospasm together with a rise in sputum eosinophil counts. Afterwards allergen sputum cells from allergic asthmatics displayed a rise in their production of IL-4 (P < 0.01), IL-6 (P < 0.05) and IL- 10 (P < 0.05) when compared to saline. By this time sputum generation of IL- 4 in atopic asthmatics was greater than in healthy subjects (P < 0.001). Furthermore, in allergic asthmatics there was a strong correlation between the rise in interleukin-4 production from sputum cells and the rise in sputum eosinophils (r = 0.87, P < 0.001). Conclusions: Sputum cell culture is a useful model to assess cytokine production in allergic asthmatics who show a marked up-regulation of Th2 cytokines following acute allergen exposure. The rise in sputum eosinophil count following allergen challenge strongly correlates with the rise in IL-4 generation from sputum cells.
http://hdl.handle.net/2268/12022
also: http://hdl.handle.net/2268/27762
http://www3.interscience.wiley.com/journal/118912028/issue

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