ORBi: Toppets Vinciane - Features of follicular dendritic cells in ovine pharyngeal tonsil: An in vivo and in vitro study in the context of scrapie pathogenesis

Reference : Features of follicular dendritic cells in ovine pharyngeal tonsil: An in vivo and in vit...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Anatomy (cytology, histology, embryology...) & physiology
	To cite this reference:	http://hdl.handle.net/2268/119836

Title :	Features of follicular dendritic cells in ovine pharyngeal tonsil: An in vivo and in vitro study in the context of scrapie pathogenesis
Language :	English
Author, co-author :	Toppets, Vinciane [Université de Liège - ULg > Département de morphologie et pathologie > Histologie >]
	Defaweux, Valérie [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
	Piret, Joëlle [Université de Liège - ULg > Département de morphologie et pathologie > Département de morphologie et pathologie >]
	Kirschvinkc, Nathalie [> >]
	Grobet, Luc [Université de Liège - ULg > Département de morphologie et pathologie > Embryologie >]
	Antoine, Nadine [Université de Liège - ULg > Département de morphologie et pathologie > Histologie >]
Publication date :	31-Jan-2011
Journal title :	Veterinary Immunology and Immunopathology
Publisher :	Elsevier Science
Volume :	141
Pages :	26-32
Audience :	International
ISSN :	0165-2427
City :	Amsterdam
Country :	The Netherlands
Keywords :	[en] Sheep ; Scrapie ; Pharyngeal tonsil ; Follicular dendritic cells ; Anti-FDC antibodies
Abstract :	[en] Although the alimentary tract has been suggested as the most likely portal of entry in natural scrapie, a growing amount of data indicates that the respiratory system and more specifically the pharyngeal tonsils serve as a natural portal of entry for scrapie. This study describes for the first time the broad cell populations in the lymphoid compartment of pharyngeal tonsils and more specifically inside the lymphoid follicles where the scrapie agent accumulates during the period of latency. Follicular dendritic cells (FDCs), stromal cells located in the light zone of the germinal centre of lymphoid follicles, seem to be the principal causal factor in the accumulation of the infectious agent in transmissible spongiform encephalopathy (TSE) diseases. Knowing that efficient lymphoreticular prion propagation requires PrPc expression, we analysed the expression of PrPc with the mouse monoclonal antibody Pri 909 both in situ and on FDC-cluster-enriched cell suspensions. In situ, a positive staining was observed in the germinal centre of pharyngeal lymph follicles. The germinal centre labelling was due to the presence of a follicular dendritic network as revealed after immunogold staining of isolated FDC clusters. Our results suggest that the pharyngeal lymphoreticular system and more specifically PrPc expressing follicular dendritic cells could serve as a prion “reservoir” during the latency phase, thus playing a key role during the scrapie lymphoinvasion.
Permalink :	http://hdl.handle.net/2268/119836

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