Reference : Features of follicular dendritic cells in ovine pharyngeal tonsil: An in vivo and in vit...
Scientific journals : Article
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
http://hdl.handle.net/2268/119836
Features of follicular dendritic cells in ovine pharyngeal tonsil: An in vivo and in vitro study in the context of scrapie pathogenesis
English
Toppets, Vinciane mailto [Université de Liège - ULg > Département de morphologie et pathologie > Histologie >]
Defaweux, Valérie mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
Piret, Joëlle mailto [Université de Liège - ULg > Département de morphologie et pathologie > Département de morphologie et pathologie >]
Kirschvinkc, Nathalie [> >]
Grobet, Luc mailto [Université de Liège - ULg > Département de morphologie et pathologie > Embryologie >]
Antoine, Nadine mailto [Université de Liège - ULg > Département de morphologie et pathologie > Histologie >]
31-Jan-2011
Veterinary Immunology and Immunopathology
Elsevier Science
141
26-32
Yes (verified by ORBi)
International
0165-2427
Amsterdam
The Netherlands
[en] Sheep ; Scrapie ; Pharyngeal tonsil ; Follicular dendritic cells ; Anti-FDC antibodies
[en] Although the alimentary tract has been suggested as the most likely portal of entry in
natural scrapie, a growing amount of data indicates that the respiratory system and more
specifically the pharyngeal tonsils serve as a natural portal of entry for scrapie.
This study describes for the first time the broad cell populations in the lymphoid compartment
of pharyngeal tonsils and more specifically inside the lymphoid follicles where
the scrapie agent accumulates during the period of latency.
Follicular dendritic cells (FDCs), stromal cells located in the light zone of the germinal
centre of lymphoid follicles, seem to be the principal causal factor in the accumulation of
the infectious agent in transmissible spongiform encephalopathy (TSE) diseases. Knowing
that efficient lymphoreticular prion propagation requires PrPc expression, we analysed
the expression of PrPc with the mouse monoclonal antibody Pri 909 both in situ and on
FDC-cluster-enriched cell suspensions.
In situ, a positive staining was observed in the germinal centre of pharyngeal lymph
follicles. The germinal centre labelling was due to the presence of a follicular dendritic
network as revealed after immunogold staining of isolated FDC clusters. Our results suggest
that the pharyngeal lymphoreticular system and more specifically PrPc expressing follicular
dendritic cells could serve as a prion “reservoir” during the latency phase, thus playing a
key role during the scrapie lymphoinvasion.
http://hdl.handle.net/2268/119836

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