Article (Scientific journals)
Hybridization of 2,659 Clostridium Perfringens Isolates with Gene Probes for Seven Toxins (Alpha, Beta, Epsilon, Iota, Theta, Mu, and Enterotoxin) and for Sialidase
Daube, Georges; Simon, Patricia; Limbourg, Bernard et al.
1996In American Journal of Veterinary Research, 57 (4), p. 496-501
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Keywords :
Clostridium perfringens; pathotypes; Toxins; DNA-DNA hybridization; animal isolates
Abstract :
[en] OBJECTIVE--To genetically characterize Clostridium perfringens isolates for association of pathologic type with various diseases. DESIGN--Prospective study. SAMPLE POPULATION--2,659 C perfringens isolates from various nonhuman animals species, human beings, and foods. PROCEDURE--Colony hybridization with DNA probes for 7 toxin (alpha, beta, epsilon, iota (subunits a and b), theta, mu, and enterotoxin) genes and 1 sialidase gene were performed to group the isolates by pathologic type. RESULTS--Enterotoxin-negative type-A isolates were the most common (2,575/2,659), were isolated from all sources, and were separated into 5 pathologic types. In cattle and horses with enterotoxemia, essentially only these pathologic types were identified. The enterotoxin-negative isolates of types C or D each had a single pathologic type. Type-C isolates were isolated only from swine with necrotic enteritis and type-D isolates from small ruminants with enterotoxemia, except that 1 type-D isolate was also found from a healthy fish. Type-B or -E isolates were not found. Among the 47 enterotoxin-positive isolates, 5 isolates from sheep or deer were type D and the other 42 were type A. These 42 isolates were grouped into 3 pathologic types: 1 type was isolated from samples of almost all origins, but the other 2 types were found in only 5 fish, 4 human beings, and 1 dog. CONCLUSIONS AND CLINICAL RELEVANCE--Genetic characterization of these isolates allowed identification of 11 different pathologic types. This approach may be useful in molecular diagnosis and prophylaxis of clostridial disease.
Disciplines :
Microbiology
Author, co-author :
Daube, Georges  ;  Université de Liège - ULiège > Bactériologie et pathologie des maladies bactériennes
Simon, Patricia ;  Université de Liège - ULiège > Bactériologie et pathologie des maladies bactériennes
Limbourg, Bernard;  Fédération de lutte contre les maladies du bétail d'Erpent
Manteca, Christophe;  Université de Liège - ULiège > Bactériologie et pathologie des maladies bactériennes
Mainil, Jacques ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes
Kaeckenbeeck, Albert ;  Université de Liège - ULiège > Bactériologie et pathologie des maladies bactériennes
Language :
English
Title :
Hybridization of 2,659 Clostridium Perfringens Isolates with Gene Probes for Seven Toxins (Alpha, Beta, Epsilon, Iota, Theta, Mu, and Enterotoxin) and for Sialidase
Publication date :
1996
Journal title :
American Journal of Veterinary Research
ISSN :
0002-9645
eISSN :
1943-5681
Publisher :
American Veterinary Medical Association, United States - Illinois
Volume :
57
Issue :
4
Pages :
496-501
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Centre d'étude de l'entérotoxémie bovine
Funders :
IRSIA - Institut pour l'Encouragement de la Recherche Scientifique dans l'Industrie et l'Agriculture [BE]
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