Reference : Estrogen Receptor-Beta in Quail: Cloning, Tissue Expression and Neuroanatomical Distr...
Scientific journals : Article
Life sciences : Zoology
Social & behavioral sciences, psychology : Neurosciences & behavior
Human health sciences : Endocrinology, metabolism & nutrition
http://hdl.handle.net/2268/11954
Estrogen Receptor-Beta in Quail: Cloning, Tissue Expression and Neuroanatomical Distribution
English
Foidart, Agnès mailto [Université de Liège - ULg > Services généraux (Faculté de médecine) > Service administratif de la Faculté (Médecine) >]
Lakaye, Bernard mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique >]
Grisar, Thierry mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique]
Ball, G. F. [> > > >]
Balthazart, Jacques mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biologie de la différenciation sexuelle du cerveau >]
5-Sep-1999
Journal of Neurobiology
40
3
327-42
Yes (verified by ORBi)
International
0022-3034
[en] A partial estrogen receptor-beta (ERbeta) cDNA had been previously cloned and sequenced in Japanese quail. The 3'- and 5'-rapid amplification of cDNA ends techniques were used here to identify a cDNA sequence of the quail ERbeta that contains a complete open reading frame. For the first time in an avian species, this cDNA sequence and the corresponding amino acid sequence are described. They are compared with the known ERbeta sequences previously described in mammals and with the ERalpha sequences identified in a selection of mammalian and avian species. The analysis by Northern blotting of the ERbeta mRNA expression in the brain and kidneys revealed the presence of several transcripts. The presence of ERbeta identified by reverse transcriptase-polymerase chain reaction demonstrated a widespread distribution quite different from the distribution of ERalpha. The complete neuroanatomical distribution of ERbeta mRNA as determined by in situ hybridization with 35S- and 33P-labeled oligoprobes is also presented. Transcripts are present in many nuclei implicated in the control of reproduction such as the medial preoptic nucleus, the nucleus striae terminalis, and the nucleus taeniae, the avian homologue of the amygdala. These data demonstrate the presence of ERbeta in a nonmammalian species and indicate that the (neuro)-anatomical distribution of this receptor type has been conserved in these two classes of vertebrates. The role of this receptor in the control of reproduction and other physiological processes should now be investigated.
http://hdl.handle.net/2268/11954

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