ORBi: Vanderghem Caroline - Effect of physicochemical characteristics of cellulosic substrates on enzymatic hydrolysis by means of a multi-stage process for cellobiose production

Reference : Effect of physicochemical characteristics of cellulosic substrates on enzymatic hydrolys...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Multidisciplinary, general & others
	To cite this reference:	http://hdl.handle.net/2268/118953

Title :	Effect of physicochemical characteristics of cellulosic substrates on enzymatic hydrolysis by means of a multi-stage process for cellobiose production
Language :	English
Author, co-author :	Vanderghem, Caroline [Université de Liège - ULg > Chimie et bio-industries > Chimie biologique industrielle >]
	Jacquet, Nicolas [Université de Liège - ULg > Chimie et bio-industries > Chimie biologique industrielle >]
	Danthine, Sabine [Université de Liège - ULg > Chimie et bio-industries > Science des alim. et formul. >]
	Blecker, Christophe [Université de Liège - ULg > Chimie et bio-industries > Science des alim. et formul. >]
	Paquot, Michel [Université de Liège - ULg > Chimie et bio-industries > Chimie biologique industrielle >]
Publication date :	Mar-2012
Journal title :	Applied Biochemistry and Biotechnology
Publisher :	Humana Press
Volume :	166
Issue/season :	6
Pages :	1423-1432
Audience :	International
ISSN :	0273-2289
City :	Totowa
Country :	NJ
Keywords :	[en] Cellobiose ; Cellulase binding ; Microcrystalline cellulose ; Multistage process ; Paper pulp ; Water retention value
Abstract :	[en] The effect of two types of cellulose, microcrystalline cellulose and paper pulp, on enzymatic hydrolysis for cellobiose production was investigated. The particle size, the relative crystallinity index and the water retention value were determined for both celluloses. A previously studied multistage hydrolysis process that proved to enhance the cellobiose production was studied with both types of celluloses. The cellobiose yield exhibited a significant improvement (120% for the microcrystalline cellulose and 75% for the paper pulp) with the multistage hydrolysis process compared to continuous hydrolysis. The conversion of cellulose to cellobiose was greater for the microcrystalline cellulose than for the paper pulp. Even with high crystallinity, microcrystalline cellulose achieved the highest cellobiose yield probably due to its highest specific surface area accessible to enzymes and quantity of adsorbed protein.
Research units :	Chimie Biologique Industrielle
Funders :	Région wallonne : Direction générale des Technologies, de la Recherche et de l'Energie - DGTRE
Name of the research project :	TECHNOSE
Target :	Researchers ; Professionals
Permalink :	http://hdl.handle.net/2268/118953
DOI :	10.1007/s12010-011-9535-1

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