Reference : Location of rRNA gene transcription sites within Ehrlich tumor cell nucleoli.
Scientific journals : Article
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
http://hdl.handle.net/2268/118299
Location of rRNA gene transcription sites within Ehrlich tumor cell nucleoli.
English
Thiry, Marc mailto [Université de Liège - ULg > Département des sciences de la vie > Biologie cellulaire >]
1991
Bulletin de l'Association des Anatomistes
Association des Anatomistes
75
229
33-7
Yes (verified by ORBi)
International
0376-6160
Nancy
France
[en] Animals ; Carcinoma, Ehrlich Tumor/genetics/ultrastructure ; Cell Nucleolus/ultrastructure ; DNA, Ribosomal/analysis/genetics/ultrastructure ; RNA, Neoplasm/genetics/ultrastructure ; RNA, Ribosomal/genetics/ultrastructure ; Transcription, Genetic/genetics
[en] The nucleolus is the morphological visualization of ribosome biogenesis. The spatial distribution of nucleolar DNA has been investigated in Ehrlich tumor cells by various immunogold labelling techniques. The DNase 1-sensitive regions within the nucleolus have been identified by in situ nick-translation at the ultrastructural level. The precise nucleolar location of rDNA and rRNA has been further specified by in situ hybridization and electron microscopy. Our results indicate that the fibrillar centers are the sole nucleolar structures where DNase 1-hypersensitive sequences, rDNA and rRNA are located together. These findings strongly suggest that rRNA gene transcription takes place within the confines of the fibrillar center, probably close to the boundary regions to the surrounding dense fibrillar component.
http://hdl.handle.net/2268/118299
also: http://hdl.handle.net/2268/29279

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