Article (Scientific journals)
Nucleolar changes and fibrillarin redistribution following apatone treatment of human bladder carcinoma cells.
Jamison, James M; Gilloteaux, Jacques; Perlaky, Laszlo et al.
2010In Journal of Histochemistry and Cytochemistry, 58 (7), p. 635-51
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Keywords :
Antineoplastic Combined Chemotherapy Protocols/pharmacology; Ascorbic Acid/pharmacology; Carcinoma/chemistry/ultrastructure; Cell Line, Tumor; Cell Nucleolus/chemistry/drug effects/ultrastructure; Chromosomal Proteins, Non-Histone/analysis; Humans; Urinary Bladder Neoplasms/chemistry/ultrastructure; Vitamin K/pharmacology; Vitamin K 3/pharmacology
Abstract :
[en] Ascorbate and menadione (Apatone) in a ratio of 100:1 kills tumor cells by autoschizis. In this study, vitamin-induced changes in nucleolar structure were evaluated as markers of autoschizis. Human bladder carcinoma (T24) cells were overlain with vitamins or with culture medium. Supernatants were removed at 1-hr intervals from 1 to 4 hr, and the cells were washed with PBS and prepared for assay. Apatone produced marked alterations in nucleolar structure including redistribution of nucleolar components, formation of ring-shaped nucleoli, condensation and increase of the proportion of perinucleolar chromatin, and the enlargement of nucleolar fibrillar centers. Immunogold labeling of the nucleolar rRNA revealed a granular localization in treated and sham-treated cells, and immunogold labeling of the rDNA revealed a shift from the fibrillar centers to the condensed perinucleolar chromatin. Fibrillarin staining shifted from the fibrillar centers and adjacent regions to a more homogeneous staining of the entire nucleolus and was consistent with the percentage of autoschizic cells detected by flow cytometry. Because autoschizis entails sequential reactivation of DNase I and DNase II, and because the fibrillarin redistribution following DNase I and Apatone treatment is identical, it appears that the nucleolar and fibrillarin changes are markers of autoschizis.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Jamison, James M
Gilloteaux, Jacques
Perlaky, Laszlo
Thiry, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire
Smetana, Karel
Neal, Deborah
McGuire, Karen
Summers, Jack L
Language :
English
Title :
Nucleolar changes and fibrillarin redistribution following apatone treatment of human bladder carcinoma cells.
Publication date :
2010
Journal title :
Journal of Histochemistry and Cytochemistry
ISSN :
0022-1554
eISSN :
1551-5044
Publisher :
Histochemical Society, New York, United States - New York
Volume :
58
Issue :
7
Pages :
635-51
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 28 April 2012

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