Reference : Feeding Cyprinus carpio with infectious materials mediates cyprinid herpesvirus 3 entry ...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/117943
Feeding Cyprinus carpio with infectious materials mediates cyprinid herpesvirus 3 entry through infection of pharyngeal periodontal mucosa
English
Fournier, Guillaume mailto [Université de Liège - ULg > > Immunologie et vaccinologie >]
Boutier, Maxime mailto [Université de Liège - ULg > > Immunologie et vaccinologie >]
Victor, Stalin Raj mailto [> >]
Mast, Jan [> >]
Parmentier, Eric mailto [Université de Liège - ULg > Département de Biologie, Ecologie et Evolution > Morphologie fonctionnelle et évolutive >]
Vanderwalle, Pierre mailto [> >]
Peeters, Dominique mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Médecine interne des animaux de compagnie >]
Lieffrig, François mailto [> >]
Farnir, Frédéric mailto [Université de Liège - ULg > Département de productions animales > Biostatistiques et bioinformatique appliquées aux sc. vétér. >]
Gillet, Laurent mailto [Université de Liège - ULg > > Immunologie et vaccinologie >]
Vanderplasschen, Alain mailto [Université de Liège - ULg > > Immunologie et vaccinologie >]
25-Jan-2012
Veterinary Research
EDP Sciences
43
6
Yes (verified by ORBi)
International
0928-4249
Les Ulis
France
[en] koi herpesvirus ; Cyprinid herpesvirus 3 ; portal of entry ; Cyprinus carpio
[en] Cyprinid herpesvirus 3 (CyHV-3), also known as Koi herpesvirus, is the etiological agent of a mortal disease in common and koi carp. Recently, we investigated the entry of CyHV-3 in carp using bioluminescence imaging and a CyHV-3 recombinant strain expressing luciferase (LUC). We demonstrated that the skin is the major portal of entry after inoculation of carp by immersion in water containing CyHV-3. While this model of infection mimics some natural conditions in which infection takes place, other epidemiological conditions could favour entry of virus through the digestive tract. Here, we investigated whether ingestion of infectious materials mediates CyHV-3 entry through the digestive tract. Carp were fed with materials contaminated with the CyHV-3 LUC recombinant (oral contamination) or immersed in water containing the virus (contamination by immersion). Bioluminescence imaging analyses performed at different times post-infection led to the following observations: (i) the pharyngeal periodontal mucosa is the major portal of entry after oral contamination, while the skin is the major portal of entry after contamination by immersion. (ii) Both modes of inoculation led to the spreading of the infection to the various organs tested. However, the timing and the sequence in which some of the organs turned positive were different between the two modes of inoculation. Finally, we compared the disease induced by the two inoculation modes. They led to comparable clinical signs and mortality rate. The results of the present study suggest that, based on epidemiological conditions, CyHV-3 can enter carp either by skin or periodontal pharyngeal mucosal infection.
Applied and Fundamental FISH Research Center - AFFISH-RC
FRIA (Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture) ; ULG (Université de Liège) ; FNRS (Fonds National Belge de la Recherche Scientifique)
http://hdl.handle.net/2268/117943
lien vers l'article publié : http://www.veterinaryresearch.org/content/43/1/6

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