Périlleux, Claire[Université de Liège - ULg > Département des sciences de la vie > Physiologie végétale >]
Tocquin, Pierre[Université de Liège - ULg > Département des sciences de la vie > Physiologie végétale >]
Du 18 avril 2012 au 19 avril 2012
[en] rhizosecretion ; proteases ; plant
[en] Plants are promising hosts for the production of complex recombinant pharmaceuticals, such as antibodies (mAbs), because they offer an inexpensive and safer alternative to traditional production systems. The plant-based production of mAbs, which are multimeric glycoproteins, require their targeting to the secretory pathaway where they are properly folded and matured. However, co-secretion of endogenous proteases, which can represent up to 10% of the extracellular proteins (secretome), is known to significantly alter the yield and quality of secreted mAbs. In this study, we analyzed the proteolytic activities in root-secretome of Arabidopsis thaliana and Nicotiana tabacum. Root-secretomes were recovered by salt extraction and the protease activity was assayed in vitro or by zymography, in a range of pH. The relative contribution of protease classes was evaluated with specific inhibitors.
Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA ; Service Public de Wallonie (Grant Waleo3 08/1/6861)
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