Reference : Degradation of recombinant IgG by root-secreted proteases of Arabidopsis thaliana and...
Scientific congresses and symposiums : Poster
Life sciences : Biochemistry, biophysics & molecular biology
Life sciences : Phytobiology (plant sciences, forestry, mycology...)
http://hdl.handle.net/2268/117243
Degradation of recombinant IgG by root-secreted proteases of Arabidopsis thaliana and Nicotiana tabacum
English
[fr] Dégradation d'IgG recombinant par les protéases sécrétées par les racine chez Arabidopsis thaliana and Nicotiana tabacum
Désiron, Carole mailto [Université de Liège - ULg > Département des sciences de la vie > Physiologie végétale >]
Lallemand, Jérôme mailto [Université de Liège - ULg > > > JCMS - FSC - Conseil Fac.]
Périlleux, Claire mailto [Université de Liège - ULg > Département des sciences de la vie > Physiologie végétale >]
Tocquin, Pierre mailto [Université de Liège - ULg > Département des sciences de la vie > Physiologie végétale >]
18-Apr-2012
A0
No
No
International
Biomedica 2012
Du 18 avril 2012 au 19 avril 2012
Liège
Belgium
[en] rhizosecretion ; proteases ; plant
[en] Plants are promising hosts for the production of complex recombinant pharmaceuticals, such as antibodies (mAbs), because they offer an inexpensive and safer alternative to traditional production systems. The plant-based production of mAbs, which are multimeric glycoproteins, require their targeting to the secretory pathaway where they are properly folded and matured. However, co-secretion of endogenous proteases, which can represent up to 10% of the extracellular proteins (secretome), is known to significantly alter the yield and quality of secreted mAbs. In this study, we analyzed the proteolytic activities in root-secretome of Arabidopsis thaliana and Nicotiana tabacum. Root-secretomes were recovered by salt extraction and the protease activity was assayed in vitro or by zymography, in a range of pH. The relative contribution of protease classes was evaluated with specific inhibitors.
Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA ; Service Public de Wallonie (Grant Waleo3 08/1/6861)
Researchers ; Professionals ; Students ; General public ; Others
http://hdl.handle.net/2268/117243

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
Poster Biomedica 2012.pdfAuthor postprint5.2 MBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.