ORBi: Désiron Carole - Degradation of recombinant IgG by root-secreted proteases of Arabidopsis thaliana and Nicotiana tabacum

Reference : Degradation of recombinant IgG by root-secreted proteases of Arabidopsis thaliana and Ni...


	Document type :	Scientific congresses and symposiums : Poster
	Discipline(s) :	Life sciences : Phytobiology (plant sciences, forestry, mycology...) Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/117243

Title :	Degradation of recombinant IgG by root-secreted proteases of Arabidopsis thaliana and Nicotiana tabacum
Language :	English
Alternative title :	[fr] Dégradation d'IgG recombinant par les protéases sécrétées par les racine chez Arabidopsis thaliana and Nicotiana tabacum
Author, co-author :	Désiron, Carole [Université de Liège - ULg > Département des sciences de la vie > Physiologie végétale >]
	Lallemand, Jérôme [Université de Liège - ULg > > > JCMS - FSC - Conseil Fac.]
	Périlleux, Claire [Université de Liège - ULg > Département des sciences de la vie > Physiologie végétale >]
	Tocquin, Pierre [Université de Liège - ULg > Département des sciences de la vie > Physiologie végétale >]
Publication date :	18-Apr-2012
Page number :	A0
On invitation :	No
Audience :	International
Event name :	Biomedica 2012
Event date :	Du 18 avril 2012 au 19 avril 2012
Event place (city) :	Liège
Event country :	Belgium
Keywords :	[en] rhizosecretion ; proteases ; plant
Abstract :	[en] Plants are promising hosts for the production of complex recombinant pharmaceuticals, such as antibodies (mAbs), because they offer an inexpensive and safer alternative to traditional production systems. The plant-based production of mAbs, which are multimeric glycoproteins, require their targeting to the secretory pathaway where they are properly folded and matured. However, co-secretion of endogenous proteases, which can represent up to 10% of the extracellular proteins (secretome), is known to significantly alter the yield and quality of secreted mAbs. In this study, we analyzed the proteolytic activities in root-secretome of Arabidopsis thaliana and Nicotiana tabacum. Root-secretomes were recovered by salt extraction and the protease activity was assayed in vitro or by zymography, in a range of pH. The relative contribution of protease classes was evaluated with specific inhibitors.
Funders :	Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA ; Service Public de Wallonie (Grant Waleo3 08/1/6861)
Target :	Researchers ; Professionals ; Students ; General public ; Others
Permalink :	http://hdl.handle.net/2268/117243

File(s) associated to this reference

Fulltext file(s):

	File	Commentary	Version	Size	Access
Restricted access	Poster Biomedica 2012.pdf		Author postprint	5.2 MB	Request copy

All documents in ORBi are protected by a user license.

Dspace v1.5