Reference : Optimization of recombinant root-secreted IgGs production in Arabidopsis thaliana by ...
Scientific congresses and symposiums : Poster
Life sciences : Phytobiology (plant sciences, forestry, mycology...)
http://hdl.handle.net/2268/117232
Optimization of recombinant root-secreted IgGs production in Arabidopsis thaliana by screening cell wall mutants
English
Boulanger, Benoit mailto [Université de Liège - ULg > > > 2e an. master bioch. & biol. moléc. & cell., fin. appr.]
Périlleux, Claire mailto [Université de Liège - ULg > Département des sciences de la vie > Physiologie végétale >]
Tocquin, Pierre mailto [Université de Liège - ULg > Département des sciences de la vie > Physiologie végétale >]
18-Apr-2012
A0
No
No
International
Biomedica 2012
du 18 au 19 avril 2012
Liège
Belgium
[en] Recombinant proteins ; Plant-based production ; Cell wall ; Root
[en] The production of complex heterologous proteins (e.g. monoclonal antibodies, mAbs) in plants has several advantages animal based systems such as low cost, scalability and limited risk of contamination by human pathogens. mAbs are glycoproteins that require to be targeted to the plant secretory pathway in order to be properly folded and matured. They are ultimately delivered in the cell wall and are expected to be freely released in the extracellular space and the external medium, which would greatly simplify downstream processing. However, a significant part of plant produced and secreted mAbs remains bound to the cell wall, therefore hindering recovery. In this study, we evaluated the extra-cellular release of root-secreted proteins of wild-type plants and cell wall mutants of Arabidopsis thaliana. Recovered protein were either analyzed by SDS-PAGE for full proteome profiling or by gelatin zymography to reveal the activity of cell wall-bound proteases. The production, secretion and release of recombinant IgG will be eventually studied in transgenic hairy-roots generated from selected mutants.
Service public de Wallonie - Waleo3 - Convention 08/1/6861
Un anticorps monoclonal matrice optimisé pour la production en cellules végétales et pour l’immobilisation en surface
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/117232

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