Reference : IMPACT OF DEPTH AND SOIL COMPACTION ON BACTERIAL DIVERSITY IN SOIL
Scientific congresses and symposiums : Poster
Life sciences : Microbiology
http://hdl.handle.net/2268/116897
IMPACT OF DEPTH AND SOIL COMPACTION ON BACTERIAL DIVERSITY IN SOIL
English
[en] Evaluating the impact of depth, compaction and soil management on the bacterial quantity and diversity with molecular methods
Stroobants, Aurore mailto [Université de Liège - ULg > Chimie et bio-industries > Biologie animale et microbienne >]
Degrune, Florine mailto [> >]
Olivier, Claire mailto [> >]
Roisin, Christian mailto [> >]
Bodson, Bernard mailto [Université de Liège - ULg > Sciences agronomiques > Phytotechnie des régions tempérées >]
Portetelle, Daniel mailto [Université de Liège - ULg > Chimie et bio-industries > Biologie animale et microbienne >]
Vandenbol, Micheline mailto [Université de Liège - ULg > Chimie et bio-industries > Biologie animale et microbienne >]
19-Aug-2012
Yes
International
14th International Symposium on Microbial Ecology
from 19-08-2012 to 24-08-2012
Copenhagen
Denmark
[en] soil ; bacteria ; depth ; compaction ; qPCR ; diversity ; tillage ; compaction
[en] Bacteria are the most abundant and diverse microorganisms in soils. The amount of bacteria in soils can reach 10^10 cells per gram of soil. These organisms are involved in various processes in agroecosystems such as nutrient cycling, contributing to plant nutrition, plant health and soil structure. The knowledge about this diversity is limited because only one percent of these organisms can be cultured by laboratory methods. During the last decades, many molecular-based techniques have been developed to assess the diversity of bacterial communities. The aim of this study was to determine the quantity and diversity of bacteria in two agricultural soils with differents soil management practices (tillage and no tillage) at different depths (10, 30 and 45 centimeters) and different compaction levels (high and low). Quantity was evaluated by real time PCR and diversity was analysed by the DGGE (Denaturing Gradient Gel Electrophoresis) technique. The results show that soil management has an impact on bacterial quantity at 45 centimeters and quantity is higher in till soil. Compaction level affects the bacterial quantity in till soil, quantity is higher in low compaction. And finally, depth influences the bacterial quantity in till and no till soil. In both soils, quantity decreases with the depth. The results will be presented and discussed on the poster.
SOLRESIDUS
http://hdl.handle.net/2268/116897

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