Reference : Zebrafish sox9b is crucial for hepatopancreatic duct development and pancreatic endocrin...
Scientific journals : Article
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
http://hdl.handle.net/2268/116882
Zebrafish sox9b is crucial for hepatopancreatic duct development and pancreatic endocrine cell regeneration
English
Manfroid, Isabelle mailto [Université de Liège - ULg > > GIGA-Research >]
Ghaye, Aurélie mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Naye, François [ > > ]
Detry, Nathalie mailto [Université de Liège - ULg > Département des sciences de la vie > Département des sciences de la vie >]
Palm, Sarah [ > > ]
Pan, Luyuan [ > > ]
Ma, Taylur [ > > ]
Huang, Wei [ > > ]
Rovira, Meritxell [ > > ]
Martial, Joseph mailto [Université de Liège - ULg > Département des sciences de la vie > Département des sciences de la vie >]
Parsons, Michael [ > > ]
Moens, Cecilia [ > > ]
Voz, Marianne mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Peers, Bernard mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
2012
Developmental Biology
Academic Press
Yes (verified by ORBi)
International
0012-1606
1095-564X
Orlando
FL
[en] Recent zebrafish studies have shown that the late appearing pancreatic endocrine cells derive from
pancreatic ducts but the regulatory factors involved are still largely unknown. Here, we show that
the zebrafish sox9b gene is expressed in pancreatic ducts where it labels the pancreatic Notchresponsive
cells previously shown to be progenitors. Inactivation of sox9b disturbs duct formation
and impairs regeneration of beta cells from these ducts in larvae. sox9b expression in the midtrunk
endoderm appears at the junction of the hepatic and ventral pancreatic buds and, by the end of
embryogenesis, labels the hepatopancreatic ductal system as well as the intrapancreatic and
intrahepatic ducts. Ductal morphogenesis and differentiation are specifically disrupted in sox9b
mutants, with the dysmorphic hepatopancreatic ducts containing misdifferentiated hepatocyte-like
and pancreatic-like cells. We also show that maintenance of sox9b expression in the extrapancreatic
and intrapancreatic ducts requires FGF and Notch activity, respectively, both pathways known to
prevent excessive endocrine differentiation in these ducts. Furthermore, beta cell recovery after
specific ablation is severely compromised in sox9b mutant larvae. Our data position sox9b as a key
player in the generation of secondary endocrine cells deriving from pancreatic ducts in zebrafish.
http://hdl.handle.net/2268/116882

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
Manfroid_Dev_Biol_2012.pdfAuthor preprint2.37 MBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.