Article (Scientific journals)
Immunocytochemical localization of ionotropic glutamate receptors subunits in the adult quail forebrain
Cornil, Charlotte; Foidart, Agnès; Minet, Arlette et al.
2000In Journal of Comparative Neurology, 428, p. 577-608
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Keywords :
AMPA; Kainate; NMDA; Japanese Quail; Coturnix japonica; aromatase; estrogen synthase
Abstract :
[en] The excitatory amino acid glutamate is implicated in the central control of many neuroendocrine and behavioral processes. The ionotropic glutamate receptors are usually divided into the N-methyl-D-aspartate (NMDA) and non-NMDA (kainate and AMPA) subtypes. Subunits of these receptors have been cloned in a few mammalian species. Information available in birds is more limited. In quail, we recently demonstrated that glutamate agonists (kainate, AMPA, and NMDA) rapidly (within minutes) and reversibly decrease in vitro aromatase activity like several other manipulations affecting intracellular HCa21 pools. Aromatase catalyzes the conversion of androgens into estrogens which is a limiting step in the control by testosterone of many behavioral and physiologic processes. Therefore, glutamate could control estrogen production in the brain, but the anatomic substrate supporting this effect is poorly understood. In quail, aromatase is mainly localized in the preoptic-hypothalamic-limbic system. We visualized here the distribution of the major ionotropic glutamate receptors in quail by immunocytochemical methods by using commercial primary antibodies raised against rat glutamate receptor 1 and receptors 2-3 (GluR1, GluR2/3: AMPA subtype, Chemicon, CA), rat glutamate receptors 5-7 (GluR5-7: kainate subtype, Pharmingen, CA), and rat NMDA receptors (NMDAR1, Pharmingen, CA). Dense and specific signals were obtained with all antibodies. The four types of receptors are broadly distributed in the brain, and, in particular, immunoreactive cells are identified within the major aromatase cell groups located in the medial preoptic nucleus, ventromedial hypothalamus, nucleus striae terminalis, and nucleus taeniae. Dense specific populations of glutamate receptor immunoreactive cells are also present with a receptor subtype-specific distribution in broad areas of the telencephalon. The distribution of glutamate receptors, therefore, is consistent with the idea that these receptors could be located at the surface of aromatase-containing cells and mediate the rapid regulation of aromatase activity in a direct manner.
Research center :
Centre de Neurobiologie Cellulaire et Moléculaire
Disciplines :
Neurosciences & behavior
Author, co-author :
Cornil, Charlotte  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie de la différenciation sexuelle du cerveau
Foidart, Agnès ;  Université de Liège - ULiège > Services généraux (Faculté de médecine) > Service administratif de la Faculté (Médecine)
Minet, Arlette ;  Centre Hospitalier Universitaire de Liège - CHU > Neurologie Sart Tilman
Balthazart, Jacques  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie de la différenciation sexuelle du cerveau
Language :
English
Title :
Immunocytochemical localization of ionotropic glutamate receptors subunits in the adult quail forebrain
Publication date :
2000
Journal title :
Journal of Comparative Neurology
ISSN :
0021-9967
eISSN :
1096-9861
Publisher :
Wiley Liss, Inc., New York, United States - New York
Volume :
428
Pages :
577-608
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
NIH - National Institutes of Health [US-MD] [US-MD]
FRFC - Fonds de la Recherche Fondamentale Collective [BE]
FWB - Fédération Wallonie-Bruxelles [BE]
ULiège FSR - Université de Liège. Fonds spéciaux pour la recherche [BE]
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