Reference : Typing of O26 enterohaemorrhagic and enteropathogenic Escherichia coli isolated from ...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
Life sciences : Microbiology
http://hdl.handle.net/2268/116010
Typing of O26 enterohaemorrhagic and enteropathogenic Escherichia coli isolated from humans and cattle with IS621 multiplex PCR-based fingerprinting.
English
Mainil, Jacques mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes]
Bardiau, Marjorie mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes >]
Ooka, T. [> > > >]
Ogura, Y. [> > > >]
Murase, K. [> > > >]
Etoh, Y. [> > > >]
Ichihara, S. [> > > >]
Horikawa, K. [> > > >]
Buvens, G. [> > > >]
Pierard, D. [> > > >]
Itoh, T. [> > > >]
Hayashi, T. [Tokyo Institute of Technology > > > >]
2011
Journal of Applied Microbiology
Blackwell Publishing
111
3
773-86
Yes (verified by ORBi)
International
1364-5072
1365-2672
Oxford
United Kingdom
[en] AIMS: This study evaluated a typing method of O26:H11 enterohaemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC) based on the variation in genomic location and copy numbers of IS621. METHODS AND RESULTS: Two multiplex PCRs, targeting either the left (5') or right (3') IS/chromosome junction of 12 IS621 insertion sites and one PCR specific of another truncated copy, were developed. Thirty-eight amplification profiles were observed amongst a collection of 69 human and bovine O26:H11 EHEC and EPEC. Seventy-one per cent of the 45 EHEC and EPEC with identical IS621 fingerprints within groups of two, three or four isolates had >85% pulsed field gel electrophoresis (PFGE) profile similarity, including four groups of epidemiologically related EHEC or EPEC, while most of the groups had <85% similarity between each others. Epidemiologically related EHEC from each of three independent outbreaks in Japan and Belgium also exhibited identical IS621 fingerprints and PFGE profiles. CONCLUSIONS: The IS621 fingerprinting and the PFGE are complementary typing assays of EHEC and EPEC; though, the former is less discriminatory. SIGNIFICANCE AND IMPACT OF THE STUDY: The IS621 printing method represents a rapid (24 h) first-line surveillance and typing assay, to compare and trace back O26:H11 EHEC and EPEC during surveys in farms, multiple human cases and outbreaks.
http://hdl.handle.net/2268/116010
also: http://hdl.handle.net/2268/135721
10.1111/j.1365-2672.2011.05089.x
Journal of Applied Microbiology (c) 2011 The Society for Applied Microbiology. No claim to Belgian Government works.

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