[en] BACKGROUND: Enteropathogenic (EPEC) and enterohaemorrhagic (EHEC) Escherichia coli are responsible for food poisoning (enteritis and enterotoxaemia) in humans in developed countries. Cattle are considered to be an important reservoir of EHEC and EPEC strains for humans. Moreover, some of the strains, belonging to the O26, O111, O118 serogroups, for example, are also responsible for digestive disorders in calves. The Translocated intimin receptor (Tir), the intimin (Eae) and the Tir-cytoskeleton coupling protein (TccP) represent three virulence factors implicated in the intimate attachment of the bacteria to the eukaryotic cell. Major variants have already been described for these genes among the different serogroups but minor variations have not often been studied. In this study, we examined the polymorphisms of the tir, eae and tccP2 genes of O26 strains (EPEC and EHEC isolated from bovines and from humans) with the aim to determine whether these polymorphisms are host specific or not. RESULTS: Of the 70 tested strains, 10 strains (14% of the strains) presented one or several polymorphisms in the tir and eae genes, which have never previously been described. Concerning tccP2 detection, 47 of the 70 strains (67% of the strains) were found to be positive for this gene. Most of the strains were found to possess tccP2 variants described in strains of serogroup O26. Nevertheless, three strains had tccP2 genes respectively described in strains of serogroup O111, O103 and O55. Moreover, none of the polymorphisms was statistically specific to the bovine or the human isolates. Nevertheless, the two major variants of tccP2 were statistically associated with the pathotype (EPEC or EHEC). CONCLUSIONS: In conclusion, tir and eae gene polymorphisms were found not to be numerous and not to be predominantly synonymous. Moreover, no difference was observed between human and bovine strains regarding the presence of polymorphisms. Finally, some tccP2 variants appeared to be pathotype specific. Further investigations need to be performed on a larger number of strains in order to confirm this specificity.
Disciplines :
Veterinary medicine & animal health Microbiology
Author, co-author :
Bardiau, Marjorie ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes
Labrozzo, S.
Mainil, Jacques ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes
Language :
English
Title :
Study of polymorphisms in tir, eae and tccP2 genes in enterohaemorrhagic and enteropathogenic Escherichia coli of serogroup O26.
Enteropathogenic Escherichia coli: unravelling pathogenesis. HD Chen G Frankel, FEMS Microbiol Rev 2005 29 83 98 10.1016/j.femsre.2004.07.002 15652977 (Pubitemid 40103580)
Prevalence and molecular typing of attaching and effacing Escherichia coli among calf populations in Belgium. B China V Pirson J Mainil, Vet Microbiol 1998 63 249 259 10.1016/S0378-1135(98)00237-5 9851003 (Pubitemid 28535141)
Laboratory investigation of hemorrhagic colitis outbreaks associated with a rare Escherichia coli serotype. JG Wells BR Davis IK Wachsmuth LW Riley RS Remis R Sokolow GK Morris, J Clin Microbiol 1983 18 512 520 6355145 (Pubitemid 13050570)
Outbreak of Enterohemorrhagic Escherichia coli O26 in Niigata City, Japan. Y Miyajima M Takahashi H Eguchi M Honma S Tanahashi Y Matui G Kobayashi M Tanaka T Higuchi Y Takeuchi, Jpn J Infect Dis 2007 60 238 239 17642545 (Pubitemid 47365053)
An enterohemorrhagic Escherichia coli O26 outbreak at a nursery school in Miyazaki, Japan. C Sonoda A Tagami D Nagatomo S Yamada R Fuchiwaki M Haruyama Y Nakamura K Kawano M Okada Y Shioyama,, et al. Jpn J Infect Dis 2008 61 92 93 18219147 (Pubitemid 351338543)
An outbreak of Escherichia coli O157 phage type 2 infection in Paisley, Scotland. A Stirling G McCartney S Ahmed J Cowden, Euro Surveill 2007 12 070823 070821
A large Escherichia coli O157 outbreak in Sweden associated with locally produced lettuce. A Soderstrom P Osterberg A Lindqvist B Jonsson A Lindberg S Blide Ulander C Welinder-Olsson S Lofdahl B Kaijser B De Jong,, et al. Foodborne Pathog Dis 2008 5 339 349 10.1089/fpd.2007.0065 18767979 (Pubitemid 351873420)
A family outbreak of Escherichia coli O157 haemorrhagic colitis caused by pork meat salami. G Conedera E Mattiazzi F Russo E Chiesa I Scorzato S Grandesso A Bessegato A Fioravanti A Caprioli, Epidemiol Infect 2007 135 311 314 10.1017/S0950268806006807 17291366 (Pubitemid 46322070)
Enterohaemorrhagic Escherichia coli O26:H11/H-: a human pathogen in emergence. M Bielaszewska W Zhang A Mellmann H Karch, Berl Munch Tierarztl Wochenschr 2007 120 279 287 17715820 (Pubitemid 47074891)
Enterohemorrhagic Escherichia coli O26:H11-Associated Hemolytic Uremic Syndrome: Bacteriology and Clinical Presentation. LB Zimmerhackl A Rosales J Hofer M Riedl T Jungraithmayr A Mellmann M Bielaszewska H Karch, Semin Thromb Hemost 2010 36 586 593 10.1055/s-0030-1262880 20865635
Child care-associated outbreak of Escherichia coli O157:H7 and hemolytic uremic syndrome. RM Raffaelli M Paladini H Hanson L Kornstein A Agasan S Slavinski D Weiss GJ Fennelly JT Flynn, Pediatr Infect Dis J 2007 26 951 953 10.1097/INF.0b013e31812571f6 17901803 (Pubitemid 47494170)
Enterohaemorrhagic Escherichia coli: emerging issues on virulence and modes of transmission. A Caprioli S Morabito H Brugère E Oswald, Vet Res 2005 36 289 311 10.1051/vetres:2005002 15845227 (Pubitemid 40716367)
Food as a vehicle for transmission of Shiga toxin-producing Escherichia coli. MC Erickson MP Doyle, J Food Prot 2007 70 2426 2449 17969631 (Pubitemid 47584242)
Verotoxigenic Escherichia coli from animals, humans and foods: who's who? JG Mainil G Daube, J Appl Microbiol 2005 98 1332 1344 10.1111/j.1365-2672.2005. 02653.x 15916647 (Pubitemid 40813520)
Shiga toxin-producing Escherichia coli strains from bovines: association of adhesion with carriage of eae and other genes. LH Wieler E Vieler C Erpenstein T Schlapp H Steinruck R Bauerfeind A Byomi G Baljer, J Clin Microbiol 1996 34 2980 2984 8940434 (Pubitemid 26389344)
Bovine feces from animals with gastrointestinal infections are a source of serologically diverse atypical enteropathogenic Escherichia coli and Shiga toxin-producing E. coli strains that commonly possess intimin. MA Hornitzky K Mercieca KA Bettelheim SP Djordjevic, Appl Environ Microbiol 2005 71 3405 3412 10.1128/AEM.71.7.3405-3412.2005 16000742 (Pubitemid 40980501)
Attaching-effacing Escherichia coli infections in Cattle. RA Moxley DR Smith, Vet Clin North Am Food Anim Pract 2010 26 29 56 table of contents 10.1016/j.cvfa.2009.10.011 20117541
Attaching effacing Escherichia coli and paradigms of Tir-triggered actin polymerization: getting off the pedestal. G Frankel AD Phillips, Cell Microbiol 2008 10 549 556 10.1111/j.1462-5822.2007.01103.x 18053003 (Pubitemid 351194084)
Allelic subtyping of the intimin locus (eae) of pathogenic Escherichia coli by fluorescent RFLP. DW Lacher H Steinsland TS Whittam, FEMS Microbiol Lett 2006 261 80 87 10.1111/j.1574-6968.2006.00328.x 16842363 (Pubitemid 43999705)
Identification of two new intimin types in atypical enteropathogenic Escherichia coli. M Blanco JE Blanco G Dahbi MP Alonso A Mora MA Coira C Madrid A Juarez MI Bernardez EA Gonzalez J Blanco, Int Microbiol 2006 9 103 110 16835840 (Pubitemid 44047822)
Typing of intimin (eae) genes from enteropathogenic Escherichia coli (EPEC) isolated from children with diarrhoea in Montevideo, Uruguay: identification of two novel intimin variants (muB and xiR/beta2B). M Blanco JE Blanco G Dahbi A Mora MP Alonso G Varela MP Gadea F Schelotto EA Gonzalez J Blanco, J Med Microbiol 2006 55 1165 1174 10.1099/jmm.0.46518-0 16914645 (Pubitemid 44373151)
TccP2 of O157:H7 and non-O157 enterohemorrhagic Escherichia coli (EHEC): challenging the dogma of EHEC-induced actin polymerization. Y Ogura T Ooka A Whale J Garmendia L Beutin S Tennant G Krause S Morabito I Chinen T Tobe,, et al. Infect Immun 2007 75 604 612 10.1128/IAI.01491-06 17101643 (Pubitemid 46203422)
Characterization of tccP2 carried by atypical enteropathogenic Escherichia coli. T Ooka MA Vieira Y Ogura L Beutin R La Ragione PM van Diemen MP Stevens I Aktan S Cawthraw A Best,, et al. FEMS Microbiol Lett 2007 271 126 135 10.1111/j.1574-6968.2007.00707.x 17403050 (Pubitemid 46701068)
Association of Escherichia coli O157:H7 tir polymorphisms with human infection. JL Bono JE Keen ML Clawson LM Durso MP Heaton WW Laegreid, BMC Infect Dis 2007 7 98 10.1186/1471-2334-7-98 17718910
Typing of intimin genes in human and animal enterohemorrhagic and enteropathogenic Escherichia coli: characterization of a new intimin variant. E Oswald H Schmidt S Morabito H Karch O Marches A Caprioli, Infect Immun 2000 68 64 71 10.1128/IAI.68.1.64-71.2000 10603369 (Pubitemid 30016678)
Comparison of eae, tir, espA and espB genes of bovine and human attaching and effacing Escherichia coli by multiplex polymerase chain reaction. B China F Goffaux V Pirson J Mainil, FEMS Microbiol Lett 1999 178 177 182 10.1111/j.1574-6968.1999.tb13775.x 10483737 (Pubitemid 29381506)
Shiga toxin gene loss and transfer in vitro and in vivo during enterohemorrhagic Escherichia coli O26 infection in humans. M Bielaszewska R Prager R Kock A Mellmann W Zhang H Tschape PI Tarr H Karch, Appl Environ Microbiol 2007 73 3144 3150 10.1128/AEM.02937-06 17400784 (Pubitemid 46834963)
Molecular evolution of the intimin gene in O111 clones of pathogenic Escherichia coli. CL Tarr TS Whittam, J Bacteriol 2002 184 479 487 10.1128/JB.184.2.479-487.2002 11751825 (Pubitemid 34027388)
Enterohaemorrhagic Escherichia coli Tir requires a C-terminal 12-residue peptide to initiate EspF-mediated actin assembly and harbours N-terminal sequences that influence pedestal length. KG Campellone MJ Brady JG Alamares DC Rowe BM Skehan DJ Tipper JM Leong, Cell Microbiol 2006 8 1488 1503 10.1111/j.1462-5822.2006.00728.x 16922867 (Pubitemid 44194580)
Phylogenetic classification of Escherichia coli O157:H7 strains of human and bovine origin using a novel set of nucleotide polymorphisms. ML Clawson JE Keen TP Smith LM Durso TG McDaneld RE Mandrell MA Davis JL Bono, Genome Biol 2009 10 56 10.1186/gb-2009-10-5-r56 19463166
Diverse genetic markers concordantly identify bovine origin Escherichia coli O157 genotypes underrepresented in human disease. J Whitworth Y Zhang J Bono E Pleydell N French T Besser, Appl Environ Microbiol 2010 76 361 365 10.1128/AEM.01761-09 19880648
Microarray based comparison of two Escherichia coli O157:H7 lineages. SE Dowd H Ishizaki, BMC Microbiol 2006 6 30 10.1186/1471-2180-6-30 16539702
Ancestral divergence, genome diversification, and phylogeographic variation in subpopulations of sorbitol-negative, beta-glucuronidase-negative enterohemorrhagic Escherichia coli O157. J Kim J Nietfeldt J Ju J Wise N Fegan P Desmarchelier AK Benson, J Bacteriol 2001 183 6885 6897 10.1128/JB.183.23.6885- 6897.2001 11698378 (Pubitemid 33064203)
Identification of Escherichia coli O157:H7 genomic regions conserved in strains with a genotype associated with human infection. M Steele K Ziebell Y Zhang A Benson P Konczy R Johnson V Gannon, Appl Environ Microbiol 2007 73 22 31 10.1128/AEM.00982-06 17056689 (Pubitemid 46079931)
Genome evolution in major Escherichia coli O157:H7 lineages. Y Zhang C Laing M Steele K Ziebell R Johnson AK Benson E Taboada VP Gannon, BMC Genomics 2007 8 121 10.1186/1471-2164-8-121 17506902
2F3 monoclonal antibody recognizes the O26 O-antigen moiety of the lipopolysaccharide of enterohemorrhagic Escherichia coli strain 4276. IM Szalo B Taminiau F Goffaux V Pirson J McCappin HJ Ball JG Mainil, Clin Diagn Lab Immunol 2004 11 532 537 15138178 (Pubitemid 38661419)
Putative adhesins of enteropathogenic (EPEC) and enterohemorrhagic (EHEC) Escherichia coli of serogroup O26 isolated from humans and cattle. M Bardiau S Labrozzo JG Mainil, J Clin Microbiol 2009
Typing of bovine attaching and effacing Escherichia coli by multiplex in vitro amplification of virulence-associated genes. B China V Pirson J Mainil, Appl Environ Microbiol 1996 62 3462 3465 8795238 (Pubitemid 26304142)
Detection of shiga-like toxin (stx1 and stx2), intimin (eaeA), and enterohemorrhagic Escherichia coli (EHEC) hemolysin (EHEC hlyA) genes in animal feces by multiplex PCR. PK Fagan MA Hornitzky KA Bettelheim SP Djordjevic, Appl Environ Microbiol 1999 65 868 872 9925634 (Pubitemid 29075149)
Molecular serotyping of Escherichia coli O26:H11. LM Durso JL Bono JE Keen, Appl Environ Microbiol 2005 71 4941 4944 10.1128/AEM.71.8.4941-4944.2005 16085902 (Pubitemid 41130865)