Reference : Analysis of calcium-induced effects on the conformation of fengycin
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/115056
Analysis of calcium-induced effects on the conformation of fengycin
English
Nasir, Mehmet Nail mailto [Université de Liège - ULg > Chimie et bio-industries > Chimie biologique industrielle >]
Laurent, Pascal mailto [Université de Liège - ULg > Chimie et bio-industries > Chimie biologique industrielle >]
Flore, Christelle [Université de Liège - ULg > Chimie et bio-industries > Biophysique moléc. numér. >]
Lins, Laurence mailto [Université de Liège - ULg > Chimie et bio-industries > Biophysique moléc. numér. >]
Ongena, Marc mailto [Université de Liège - ULg > Chimie et bio-industries > Bio-industries >]
Deleu, Magali mailto [Université de Liège - ULg > Chimie et bio-industries > Chimie biologique industrielle >]
2013
Spectrochimica Acta Part A : Molecular and Biomolecular Spectroscopy
Pergamon
110
450-457
Yes (verified by ORBi)
International
1386-1425
Kidlington
United Kingdom
[en] Fengycin ; Ellicitor ; Antifungal ; Conformational analysis ; Lipopeptide ; Baciullus subtilis
[en] A combination of CD, FT-IR, NMR and fluorescence spectroscopic techniques was applied to elucidate the conformation of fengycin, a natural lipopeptide with antifungal and eliciting activities, in a membrane-mimicking environment and to investigate the effect of calcium ions on the conformation.
We mainly observed that fengycin adopts a turn conformation and that the side chain of glutamate residues plays a key role on the stabilization of the peptide ring backbone conformation. More particularly, the binding of calcium ions by the carboxylic moieties has a consequence on the environment of the tyrosine residues. Our data suggest also an arrangement of fengycin molecules into “-sheet like micelles” in a membrane-mimicking environment and the enhancement of this aggregating effect in presence of calcium ions.
The modulation of the fengycin conformation by the environmental conditions may influence its biological properties.
Patrimoine de l'Université de Liège-Gembloux Agro-Bio Tech
Mode d’action et valorisation des lipopeptides et glycolipides en tant qu’agents immunostimulants chez les végétaux
Researchers ; Professionals ; Students ; General public ; Others
http://hdl.handle.net/2268/115056

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