ORBi: Nasir Mehmet Nail - Analysis of calcium-induced effects on the conformation of fengycin

Reference : Analysis of calcium-induced effects on the conformation of fengycin


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/115056

Title :	Analysis of calcium-induced effects on the conformation of fengycin
Language :	English
Author, co-author :	Nasir, Mehmet Nail [Université de Liège - ULg > Chimie et bio-industries > Chimie biologique industrielle >]
	Laurent, Pascal [Université de Liège - ULg > Chimie et bio-industries > Chimie biologique industrielle >]
	Flore, Christelle [Université de Liège - ULg > Chimie et bio-industries > Biophysique moléc. numér. >]
	Lins, Laurence [Université de Liège - ULg > Chimie et bio-industries > Biophysique moléc. numér. >]
	Ongena, Marc [Université de Liège - ULg > Chimie et bio-industries > Bio-industries >]
	Deleu, Magali [Université de Liège - ULg > Chimie et bio-industries > Chimie biologique industrielle >]
Publication date :	2013
Journal title :	Spectrochimica Acta Part A : Molecular and Biomolecular Spectroscopy
Publisher :	Pergamon
Volume :	110
Pages :	450-457
Audience :	International
ISSN :	1386-1425
City :	Kidlington
Country :	United Kingdom
Keywords :	[en] Fengycin ; Ellicitor ; Antifungal ; Conformational analysis ; Lipopeptide ; Baciullus subtilis
Abstract :	[en] A combination of CD, FT-IR, NMR and fluorescence spectroscopic techniques was applied to elucidate the conformation of fengycin, a natural lipopeptide with antifungal and eliciting activities, in a membrane-mimicking environment and to investigate the effect of calcium ions on the conformation. We mainly observed that fengycin adopts a turn conformation and that the side chain of glutamate residues plays a key role on the stabilization of the peptide ring backbone conformation. More particularly, the binding of calcium ions by the carboxylic moieties has a consequence on the environment of the tyrosine residues. Our data suggest also an arrangement of fengycin molecules into “-sheet like micelles” in a membrane-mimicking environment and the enhancement of this aggregating effect in presence of calcium ions. The modulation of the fengycin conformation by the environmental conditions may influence its biological properties.
Funders :	Patrimoine de l'Université de Liège-Gembloux Agro-Bio Tech
Name of the research project :	Mode d’action et valorisation des lipopeptides et glycolipides en tant qu’agents immunostimulants chez les végétaux
Target :	Researchers ; Professionals ; Students ; General public ; Others
Permalink :	http://hdl.handle.net/2268/115056

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