Article (Scientific journals)
Analysis of calcium-induced effects on the conformation of fengycin
Nasir, Mehmet Nail; Laurent, Pascal; Flore, Christelle et al.
2013In Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, 110, p. 450-457
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Keywords :
Fengycin; Ellicitor; Antifungal; Conformational analysis; Lipopeptide; Baciullus subtilis
Abstract :
[en] A combination of CD, FT-IR, NMR and fluorescence spectroscopic techniques was applied to elucidate the conformation of fengycin, a natural lipopeptide with antifungal and eliciting activities, in a membrane-mimicking environment and to investigate the effect of calcium ions on the conformation. We mainly observed that fengycin adopts a turn conformation and that the side chain of glutamate residues plays a key role on the stabilization of the peptide ring backbone conformation. More particularly, the binding of calcium ions by the carboxylic moieties has a consequence on the environment of the tyrosine residues. Our data suggest also an arrangement of fengycin molecules into “-sheet like micelles” in a membrane-mimicking environment and the enhancement of this aggregating effect in presence of calcium ions. The modulation of the fengycin conformation by the environmental conditions may influence its biological properties.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Nasir, Mehmet Nail ;  Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle
Laurent, Pascal ;  Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle
Flore, Christelle ;  Université de Liège - ULiège > Chimie et bio-industries > Biophysique moléc. numér.
Lins, Laurence  ;  Université de Liège - ULiège > Chimie et bio-industries > Biophysique moléc. numér.
Ongena, Marc ;  Université de Liège - ULiège > Chimie et bio-industries > Bio-industries
Deleu, Magali  ;  Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle
Language :
English
Title :
Analysis of calcium-induced effects on the conformation of fengycin
Publication date :
2013
Journal title :
Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
ISSN :
1386-1425
Publisher :
Pergamon, Kidlington, United Kingdom
Volume :
110
Pages :
450-457
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Mode d’action et valorisation des lipopeptides et glycolipides en tant qu’agents immunostimulants chez les végétaux
Funders :
ULiège. Patrimoine - Université de Liège. Patrimoine [BE]
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