[en] The surface hydrophobicity of nine Bacillus subtilis strains in different states (spores, vegetative cells, and dead cells) was assessed by water contact angle measurements, hydrophobic interaction chromatography (HIC) and bacterial adhesion to hydrocarbon (BATH). Electrokinetic properties of B, subtilis strains were characterized by zeta potential measurements and found to differ appreciably according to the strain, Correlations between HIC data, BATH data and zeta potential showed that HIC and RATH are influenced by electrostatic interactions. Water contact angle measurements thus provide a better estimate of cell surface hydrophobicity. The water contact angle of B. subtilis varied according to the strain and the state, the spores tending to be more hydrophobic than vegetative cells. (C) 2001 Elsevier Science B.V. All rights reserved.
Disciplines :
Agriculture & agronomy
Author, co-author :
Ahimou, F.
Paquot, Michel ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Absolom D.R., Lamberti F.V., Policova Z., Zingg W., Van Oss C.J., Neu A.W. (1983) Surface thermodynamics of bacterial adhesion. Appl. Environ. Microbiol. 46:90-97.
Ahimou F., Jacques Ph., Deleu M. (2000) Surfactin and iturin A effects on Bacillus subtilis surface hydrophobicity. Enzyme Microbial. Technol. 27:749-754.
Asaka O., Shoda M. (1996) Biocontrol of Rhizoctonia solani damping-off of tomato with Bacillus subtilis RB14. Appl. Environ. Microbiol. 62:4081-4085.
Busscher H.J., Weerkamp A.H., Van der Mei H.C., Van Pelt A.W.J., De Jong H.P., Arends J. (1984) Measurement of the surface free energy of bacterial cell surfaces and its relevance for adhesion. Appl. Environ. Microbiol. 48:980-983.
Busscher H.J., Van de Belt-Gritter B., Van der Mei H.C. (1995) Implications of microbial adhesion to hydrocarbons for evaluating cell surface hydrophobicity. 1. Zeta potentials of hydrocarbon droplets. Colloids Surf., B 5:111-116.
Clark W.B., Lane M.D., Beem E., Bragg S.L., Wheeler T.T. (1985) Relative hydrophobicities of Actinomyces viscosus and Actinomyces naeslundii strains and their adsorption to saliva-treated hydroxyapatite. Infect. Immun. 47:730-736.
Delcambe L. (1965) L'iturine. Préparation, purification et poids moléculaire. Bull. Soc. Chim. Bel. 74:315-328.
Dewez J.L., Berger V., Schneider Y.J., Rouxhet P.G. (1997) Influence of substrate hydrophobicity on the adsorption of collagen in the presence of pluronic F68, albumin, or calf serum. J. Colloid Interface Sci. 191:1-10.
Dillon J.K., Fuerst J.A., Hayward A.C., Davis G.H.G. (1986) A comparison of five methods for assaying bacterial hydrophobicity. J. Microbiol. Methods 6:13-19.
Garry P., Andersen T., Vendeuvre J.L., Bellon-Fontaine M.N. (1995) Influence de la rugosité de surfaces en polyuréthane sur l'adhésion de Bacillus subtilis et de Bacillus cereus. Adhésion des Micro-organismes Aux Surfaces , Bellon-Fontaine, M.-N., Fourniat, J. (Eds.). Lavoisier, Paris; 21-30.
Garry P., Vendeuvre J.L., Bellon-Fontaine M.N. (1998) Surface properties and adhesion of Bacillus cereus and Bacillus subtilis to polyurethane-influence of growth temperature. J. Dispersion Sci. Technol. 19:1175-1197.
Geertsema-Doornbusch G.I., Van der Mei H.C., Busscher H.J. (1993) Microbial cell surface hydrophobicity. The involvement of electrostatic interactions in microbial adhesion to hydrocarbons (MATH). J. Microbiol. Methods 18:61-68.
Husmark U., Rönner U. (1990) Forces involved in adhesion of Bacillus cereus spores to solid surfaces under different environmental conditions. J. Appl. Bacteriol. 69:557-562.
Kemper M.A., Urrutia M.M., Beveridge T.J., Koch A.L., Doyle R.J. (1993) Proton motive force may regulate cell wall-associated enzymes of Bacillus subtilis. J. Bacteriol. 175:5690-5696.
Kosh A.L. (1986) The pH in the neighborhood of membranes generating a proton motive force. J. Theor. Biol. 120:73-84.
Koshikawa T., Yamazaki M., Yoshimi M., Ogawa S., Yamada A., Watabe K., Torii M. (1989) Surface hydrophobicity of spores of Bacillus spp. J. Gen. Microbiol. 135:2717-2722.
Mozes N., Rouxhet P.G. (1987) Methods for measuring hydrophobicity of microorganisms. J. Microbiol. Methods 6:99-112.
Paquot M., Heuzé V., Destain J., Lepoivre Ph., Tossut P., Thonart Ph. (1994) Surface hydrophobicity and electrical properties of Bacillus subtilis strains, potential phytopathogen antagonists in tomato crops. Colloids Surf., B 2:451-456.
Razafindralambo H., Deleu M., Popineau Y., Hbid C., Jacques P., Thonart P., Paquot M. (1998) Foaming properties of lipopeptides produced by Bacillus subtilis: Effect of lipid and peptide structural attributes. J. Agric. Food Chem. 46:911-916.
Rönner U., Husmark U., Henriksson A. (1990) Adhesion of Bacillus spores in relation to hydrophobicity. J. Appl. Bacteriol. 69:550-556.
Rosenberg M., Gutnick D., Rosenberg E. (1980) Adherence of bacteria to hydrocarbons: A simple method for measuring cell-surface hydrophobicity. FEMS Microbiol. Lett. 9:29-33.
Rosenberg E., Brown D.R., Demain A.L. (1985) The influence of gramicidin S on hydrophobicity of germinating Bacillus brevis spores. Arch. Microbiol. 142:51-54.
Stenström A.T. (1989) Bacterial hydrophobicity, an overall parameter for the measurement of adhesion potential to soil particles. Appl. Environ. Microbiol. 55:142-147.
Thimon L., Peypoux F., Maget-Dana R., Michel G. (1992) Surface-active properties of antifungal lipopeptides produced by Bacillus subtilis. J. Am. Oil Chem. Soc. 69:92-93.
Van der Mei H.C., Weerkamp A.H., Busscher H.J. (1987) A comparison of various methods to determine hydrophobic properties of streptococcal cell surfaces. J. Microbiol. Methods 6:277-287.
Van der Mei H.C., Van de Belt-Gritter B., Busscher H.J. (1995) Implications of microbial adhesion to hydrocarbons for evaluating cell surface hydrophobicity: 2. Adhesion mechanisms. Colloids Surf., B 5:117-126.
Van Loosdrecht M.C.M., Lyklema J., Norde W., Schraa G., Zehnder A.J.B. (1987) The role of bacterial cell wall hydrophobicity in adhesion. Appl. Environ. Microbiol. 53:1893-1897.
Van Loosdrecht M.C.M., Lyklema J., Norde W., Schraa G., Zehnder A.J.B. (1987) Electrophoretic mobility and hydrophobicity as a measure to predict the initial steps of bacterial adhesion. Appl. Environ. Microbiol. 53:1898-1901.
Wiencek K.M., Klapes N.A., Foegeding P.M. (1990) Hydrophobicity of Bacillus and Clostridium spores. Appl. Environ. Microbiol. 56:2600-2605.
