Reference : A Temperature-sensitive mutation in the Arabidopsis thaliana phosphomannomutase gene ...
Scientific journals : Article
Life sciences : Phytobiology (plant sciences, forestry, mycology...)
http://hdl.handle.net/2268/11334
A Temperature-sensitive mutation in the Arabidopsis thaliana phosphomannomutase gene disrupts protein glycosylation and triggers cell death.
English
Hoeberichts, Frank A [Flanders Institute for Biotechnology (VIB) > Department of Plant Systems Biology > > >]
Vaeck, Elke [Flanders Institute for Biotechnology - VIB > Departement of Plant Systems Biology > > >]
Kiddle, Guy [Rothamsted Research > Crop Performance and Improvement Division > > >]
Coppens, Emmy [Flanders Institute for Biotechnology (VIB) > Department of Plant Systems Biology > > > > >]
van de Cotte, Brigitte [Flanders Institute for Biotechnology (VIB) > Department of Plant Systems Biology > > > > > >]
Adamantidis, Antoine mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique >]
Ormenese, Sandra mailto [Université de Liège - ULg > Département des sciences de la vie > Physiologie végétale > > Physiologie végétale]
Foyer, Christine H [Rothamsted Research > Crop Performance and Improvement Division > > > > > >]
Zabeau, Marc [Flanders Institute for Biotechnology (VIB) > Department of Plant Systems Biology > > > > > >]
Inze, Dirk [Flanders Institute for Biotechnology (VIB) > Department of Plant Systems Biology > > > > > >]
Périlleux, Claire mailto [Université de Liège - ULg > Département des sciences de la vie > Physiologie végétale >]
Van Breusegem, Frank mailto [Flanders Institute for Biotechnology (VIB) > Department of Plant Systems Biology > > > > > >]
Vuylsteke, Marnik [Flanders Institute for Biotechnology (VIB) > Department of Plant Systems Biology > > > > > >]
2008
Journal of Biological Chemistry
American Society for Biochemistry and Molecular Biology
283
9
5708-18
Yes (verified by ORBi)
International
0021-9258
1083-351X
Baltimore
MD
[en] Arabidopsis/enzymology/genetics ; Ascorbic Acid/biosynthesis/genetics ; Catalysis ; Cell Death/genetics ; Glycoproteins/biosynthesis/genetics ; Glycosylation ; Guanosine Diphosphate Mannose/biosynthesis/genetics ; Hot Temperature ; Mannosephosphates/biosynthesis/genetics ; Mutation ; Phenotype ; Phosphotransferases (Phosphomutases)/genetics/metabolism ; Plant Proteins/genetics/metabolism ; Protein Disulfide-Isomerases/genetics/metabolism ; Recombinant Proteins/genetics/metabolism ; Seedling/enzymology/genetics
[en] Eukaryotic phosphomannomutases (PMMs) catalyze the interconversion of mannose 6-phosphate to mannose 1-phosphate and are essential to the biosynthesis of GDP-mannose. As such, plant PMMs are involved in ascorbic acid (AsA) biosynthesis and N-glycosylation. We report on the conditional phenotype of the temperature-sensitive Arabidopsis thaliana pmm-12 mutant. Mutant seedlings were phenotypically similar to wild type seedlings when grown at 16-18 degrees C but died within several days after transfer to 28 degrees C. This phenotype was observed throughout both vegetative and reproductive development. Protein extracts derived from pmm-12 plants had lower PMM protein and enzyme activity levels. In vitro biochemical analysis of recombinant proteins showed that the mutant PMM protein was compromised in its catalytic efficiency (K cat/K m). Despite significantly decreased AsA levels in pmm-12 plants, AsA deficiency could not account for the observed phenotype. Since, at restrictive temperature, total glycoprotein patterns were altered and glycosylation of protein-disulfide isomerase was perturbed, we propose that a deficiency in protein glycosylation is responsible for the observed cell death phenotype.
Politique Scientifique Fédérale (Belgique) = Belgian Federal Science Policy ; Research Fund of the Ghent University ; Research Foundation-Flanders
Researchers
http://hdl.handle.net/2268/11334
10.1074/jbc.M704991200

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