Article (Scientific journals)
Evaluation of the cytotoxicity of organic dust components on thp1 monocytes-derived-macrophages using high content analysis
Ramery, Eve; O'Brien, Peter James
2012In Environmental Toxicology
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Keywords :
Organic dust; Toxicity; HCA
Abstract :
[en] Organic dust contains pathogen-associated molecular patterns (PAMPs) which can induce significant airway diseases following chronic exposure. Mononuclear phagocytes are key protecting cells of the respiratory tract. Several studies have investigated the effects of PAMPs and mainly endotoxins, on cytokine production. However the sub-lethal cytotoxicity of organic dust components on macrophages has not been tested yet. The novel technology of high content analysis (HCA) is already used to assess subclinical drug-induced toxicity. It combines the capabilities of flow cytometry, intracellular fluorescence probes, and image analysis and enables rapid multiple analyses in large numbers of samples. In the present study, HCA was used to investigate the cytotoxicity of the 3 major PAMPs contained in organic dust, ie. endotoxin (LPS), peptidoglycan (PGN) and β-glucans (zymosan) on THP-1 monocyte-derived macrophages. LPS was used at concentrations of 0.005, 0.01, 0.02, 0.05, 0.1 and 1 μg/ml; PGN and zymosan were used at concentrations of 1, 5, 10, 50, 100 and 500 μg/ml. Cells were exposed to PAMPs for 24 h. In addition, the oxidative burst and the phagocytic capabilities of the cells were tested. An overlap between PGN intrinsic fluorescence and red/far-red fluorescent dyes occurred, rendering the evaluation of some parameters impossible for PGN. LPS induced sub-lethal cytotoxicity at the lowest dose (from 50 ng/ml). However, the greatest cytotoxic changes occurred with zymosan. In addition, zymosan, but not LPS, induced phagosome maturation and oxidative burst. Given the fact that β-glucans can be up to 100-fold more concentrated in organic dust than LPS, these results suggest that β-glucans could play a major role in macrophage impairment following heavy dust exposure and will merit further investigation in the near future.
Research center :
UCD Dublin Veterinary Sciences Center
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Environmental sciences & ecology
Animal production & animal husbandry
Author, co-author :
Ramery, Eve ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Biochimie
O'Brien, Peter James
Language :
English
Title :
Evaluation of the cytotoxicity of organic dust components on thp1 monocytes-derived-macrophages using high content analysis
Publication date :
February 2012
Journal title :
Environmental Toxicology
ISSN :
1520-4081
eISSN :
1522-7278
Publisher :
John Wiley & Sons, Inc
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 01 March 2012

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