ORBi: Vazquez-Jimenez J. F. - Moderate hypothermia during cardiopulmonary bypass reduces myocardial cell damage and myocardial cell death related to cardiac surgery.

Reference : Moderate hypothermia during cardiopulmonary bypass reduces myocardial cell damage and my...


	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Surgery Human health sciences : Pediatrics
	To cite this reference:	http://hdl.handle.net/2268/112974

Title :	Moderate hypothermia during cardiopulmonary bypass reduces myocardial cell damage and myocardial cell death related to cardiac surgery.
Language :	English
Author, co-author :	Vazquez-Jimenez, J. F. [Rheinisch - Westfälische Technische Hochschule Aachen - RWTH > > > Thoracic and Cardiovascular Surgery > > >]
	Qing, M. [Rheinisch - Westfälische Technische Hochschule Aachen - RWTH > > > Pediatric Cardiology > > >]
	Hermanns, B. [Rheinisch - Westfälische Technische Hochschule Aachen - RWTH > > > Institute of Pathology Center for Clinical Research BIOMAT > >]
	Klosterhalfen, B. [Rheinisch - Westfälische Technische Hochschule Aachen - RWTH > > > Institute of Pathology Center for Clinical Research BIOMAT > >]
	Woltje, M. [Rheinisch - Westfälische Technische Hochschule Aachen - RWTH > > > Institute of Interdisciplinary Center for Clinical Research BIOMAT > > >]
	Chakupurakal, R. [Rheinisch - Westfälische Technische Hochschule Aachen - RWTH > > > Pediatric Cardiology > > >]
	SCHUMACHER, Katharina [Rheinisch - Westfälische Technische Hochschule Aachen - RWTH > Pediatric Cardiology > > >]
	Messmer, B. J. [Rheinisch - Westfälische Technische Hochschule Aachen - RWTH > > > Thoracic and Cardiovascular Surgery > > >]
	von Bernuth, G. [Rheinisch - Westfälische Technische Hochschule Aachen - RWTH > > > Pediatric Cardiology > > >]
	SEGHAYE, Marie-Christine [Rheinisch - Westfälische Technische Hochschule Aachen - RWTH > Pediatric Cardiology > > >]
Publication date :	2001
Journal title :	Journal of the American College of Cardiology
Publisher :	Elsevier Science
Volume :	38
Issue/season :	4
Pages :	1216-23
Audience :	International
ISSN :	0735-1097
e-ISSN :	1558-3597
City :	New York
Country :	NY
Keywords :	[en] Animals ; Apoptosis ; Cardiopulmonary Bypass ; Cell Death ; Female ; Hemodynamics ; Hypothermia, Induced ; In Situ Nick-End Labeling ; Interleukin-10/biosynthesis ; Myocardium/metabolism/pathology ; Swine ; Tumor Necrosis Factor-alpha/biosynthesis
Abstract :	[en] OBJECTIVES: The goal of this study was to test the hypothesis that moderate hypothermia during cardiopulmonary bypass (CPB) provides myocardial protection by enhancing intra-myocardial anti-inflammatory cytokine balance. BACKGROUND: Moderate hypothermia during experimental CPB stimulates production of interleukin-10 (IL10) and blunts release of tumor necrosis factor-alpha (TNFalpha). METHODS: Twelve young pigs were assigned to a temperature (T degrees ) regimen during CPB: moderate hypothermia (T degrees : 28 degrees C; n = 6) and normothermia (T degrees : 37 degrees C; n = 6). Intra-myocardial TNFalpha- and IL10-messenger RNA were detected by competitive reverse transcriptase polymerase chain reaction and quantification of cytokine synthesis by Western blot. Levels of cardiac troponin I (cTnI) in cardiac lymph and in arterial and coronary venous blood were examined during and after CPB. Myocardial cell damage was assessed by histologic and ultrastructural anomalies of tissue probes taken 6 h after CPB. RESULTS: Synthesis of IL10 was significantly higher, while that of TNFalpha was significantly lower, in pigs that were in moderate hypothermia during surgery than in the others. In contrast with normothermia, moderate hypothermia was also associated with significantly lower cumulative cardiac lymphatic flow during and after CPB, significantly lower lymphatic cTnI concentrations after CPB, significantly lower percentages of myocardial cell necrosis and a significantly lower score of ultrastructural anomalies of myocardial cells. While the percentage of apoptotic cells was not different between groups, the apoptosis/necrosis ratio tended to be higher in animals that were in moderate hypothermia during surgery. In all animals, TNFalpha synthesis correlated positively while IL10 production correlated negatively with necrosis and total cell death, respectively. CONCLUSIONS: Our results suggest that moderate hypothermia during CPB provides myocardial protection by enhancing intra-myocardial anti-inflammatory cytokine balance.
Permalink :	http://hdl.handle.net/2268/112974

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