Reference : Antioxidant and Antiradical Activities of Manihot esculenta Crantz (Euphorbiaceae) Le...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
Life sciences : Food science
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/99044
Antioxidant and Antiradical Activities of Manihot esculenta Crantz (Euphorbiaceae) Leaves and Other Selected Tropical Green Vegetables Investigated on Lipoperoxidation and Phorbol-12-myristate-13-acetate (PMA) Activated Monocytes
English
[fr] Activités antioxydantes et antiradicalaires des feuilles de Manihot esculenta (Crantz) et d'autres légumes verts tropicaux sélectionnés, investiguées sur lipoperoxydation et des monocytes activés au phorbol 12-myristate, 13-acétate (PMA)
Tsumbu, César Ndele mailto [Université de Liège - ULg > > > Form. doc. sc. bioméd. & pharma.]
Deby-Dupont, Ginette [Université de Liège - ULg > > Centre de l'Oxygène, Recherche et Développement, CORD > >]
Tits, Monique mailto [Université de Liège - ULg > Département de pharmacie > Phytochimie et phytothérapie >]
Angenot, Luc mailto [Université de Liège - ULg > Département de pharmacie > Département de pharmacie >]
Franck, Thierry mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Serteyn, Didier mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Mouithys-Mickalad, Ange mailto [Université de Liège - ULg > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
16-Sep-2011
Nutrients
Molecular Diversity Preservation International (MDPI)
3
9
818-838
Yes (verified by ORBi)
International
2072-6643
Basel
Switzerland
[en] monocytes ; lipid peroxidation ; reactive oxygen species ; green vegetable ; antioxidant ; polyphenols
[en] Abelmoschus esculentus (Malvaceae), Hibiscus acetosella (Malvaceae), Manihot esculenta Crantz (Euphorbiaceae) and Pteridium aquilinum (Dennstaedtiaceae) leaves are currently consumed as vegetables by migrants from sub-Saharan Africa living in Western Europe and by the people in the origin countries, where these plants are also used in the folk medicine. Manihot leaves are also eaten in Latin America and some Asian countries. This work investigated the capacity of aqueous extracts prepared from those vegetables to inhibit the peroxidation of a linoleic acid emulsion. Short chain, volatile C-compounds as markers of advanced lipid peroxidation were measured by gas chromatography by following the ethylene production. The generation of lipid hydroperoxides, was monitored by spectroscopy using N-N′-dimethyl-p-phenylene-diamine (DMPD). The formation of intermediate peroxyl, and other free radicals, at the initiation of the lipid peroxidation was investigated by electron spin resonance, using α-(4-pyridyl-1-oxide)-N-tert-butylnitrone as spin trap agent. The ability of the extracts to decrease the cellular production of reactive oxygen species (ROS) in “inflammation like” conditions was studied by fluorescence technique using 2′,7′-dichlorofluorescine-diacetate as fluorogenic probe, in a cell model of human monocytes (HL-60 cells) activated with phorbol ester. Overall the extracts displayed efficient concentration-dependent inhibitory effects. Their total polyphenol and flavonoid content was determined by classic colorimetric methods. An HPLC-UV/DAD analysis has clearly identified the presence of some polyphenolic compounds, which explains at least partially the inhibitions observed in our models. The role of these plants in the folk medicine by sub-Saharan peoples as well as in the prevention of oxidative stress and ROS related diseases requires further consideration.
Faculté de médecine; département de pharmacie, CIRM, laboratoire de pharmacognosie/ Faculté des sciences, centre de l'oxygène, recherche et développement (CORD)
1) SP Partnership Association Lich (Germany), 2) Biebergemünder Association for South Development” (Germany), 3) International Help Service IPHD Linz (Austria)
Researchers ; Professionals ; Students ; General public
http://hdl.handle.net/2268/99044
also: http://hdl.handle.net/2268/112629
10.3390/nu3090818

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