[en] Antioxidant status and titers of autoantibodies against oxidized low-density lipoproteins (ox-LDL-Ab) were investigated in top soccer (S; n = 21, age 24.6 +/- 4.3 years) and basketball (B; n 3,000 mIU/ml) in ox-LDL-Ab were found in half the players (12S and 4B) with a maximum reaching 6000 mIU/ml (normal range: 200-600 mIU/ml), showing an in vivo LDL oxidation. There was no correlation between ox-LDL-Ab titers and chlolesterol, LDL cholesterol, or antioxidant levels. Nevertheless, plasma vitamin C concentration was lower in athletes having high levels of ox-LDL-Ab when compared with those with normal levels (8.49 +/- 3.14 mirogram/ml vs. 10.39 +/- 2.55 microgram/ml), but this difference was not statistically significant. In conclusion, our data suggest that potential atherogenic and cardiovascular risks as reflected by high titers in ox-LDL-Ab may exist in some top athletes despite a nonaltered antioxidant status.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Pincemail, Joël ; Centre Hospitalier Universitaire de Liège - CHU > Chirurgie cardio-vasculaire
Lecomte, J.
Castiau, J.
Collard, E.
Vasankari, T.
Cheramy-Bien, Jean-Paul ; Centre Hospitalier Universitaire de Liège - CHU > Chirurgie cardio-vasculaire
Limet, Raymond ; Université de Liège - ULiège > Département des sciences cliniques > Chirurgie cardio-vasculaire et thoracique
Defraigne, Jean-Olivier ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie générale, bioch. et path. humaine et pathologiques
Language :
English
Title :
Evaluation of Autoantibodies against Oxidized Ldl and Antioxidant Status in Top Soccer and Basketball Players after 4 Months of Competition
Packer L. Oxidants, antioxidant nutrients, and the athlete. J Sport Sci. 15:1997;353-363.
Ji L.L. Exercise, oxidative stress, and antioxidants. Am. J. Sports Med. 24:1996;S20-S24.
Pincemail J., Camus G., Roesgen A., Dreezen E., Bertrand Y., Lismonde M., Deby-Dupont G., Deby C. Exercise induces pentane production and neutrophil activation in humans. Effect of propranolol. Eur. J. Appl. Physiol. 61:1990;319-322.
Marzatico, F.; Pansarasa, O.; Bertorelli, L.; Somenzini, L.; Della Valle, G. Blood free radical antioxidant enzymes and lipid peroxides following long-distance and lactacidemic performances in highly trained aerobic and sprint athletes. J. Sports Med. Phys. Fit. 37:235-239, 1997.
Poulsen H.E., Loft S., Vistisen K. Extreme exercise and oxidative DNA modification. J. Sports Med. 14:1996;343-346.
Pincemail J., Deby C., Camus G., Pirnay F., Bouchez R., Massaux L., Goutier R. Tocopherol mobilization during intensive exercise. Eur. J. Appl. Physiol. 57:1988;189-191.
Buczynski A., Kedziora J., Tkaczewski W., Wachowicz B. Effect of submaximal physical exercise on adaptative protection of human blood platelets. Int. J. Sports Med. 12:1991;52-54.
Hellsten Y., Apple F.S., Sjodin B. Effect of sprint cycle training on activities of antioxidant enzymes in human skeletal muscle. J. Appl. Physiol. 81:1996;1484-1487.
Gohil K., Viguie C., Stanley W.C., Brooks G.A., Packer L. Blood glutathione oxidation during human exercise. J. Appl. Physiol. 64:1998;115-119.
Kretzschmar M., Müller D., Hübscher J., Marin E., Klinger W. Influence of aging and acute physical exercise on plasma glutathione and lipid peroxides in man. Int. J. Sports Med. 12:1991;218-222.
Ashton T., Rowlands C.C., Jones E., Young I.S., Jackson S.K., Davies B., Peters J.R. Electron spin resonance spectroscopic detection of oxygen-centered radicals in human serum following exhaustive exercise. Eur. J. Appl. Physiol. 77:1998;498-502.
Wetzstein C.J., Shern-Brewer R.A., Santanam N., Green N., White-Welkley J.E., Parthasarathy S. Does acute exercise affect the susceptibility of low-density lipoprotein to oxidation? Free Radic. Biol. Med. 24:1998;679-682.
Vasankari T.J., Kujala U.R., Vasankari T.M., Ahotupa M. Reduced oxidized LDL levels after a 10 month exercise program. Med. Sci. Sports Exer. 30:1998;1496-1501.
Kujala U.M., Ahotupa M., Vasankari T., Kaprio J., Tikkanen M.J. Low LDL oxidation in veteran endurance athletes. Scand. J. Med. Sci. Sports. 6:1996;303-308.
Salonen J.T., Yla-Herttuala S., Yamamoto R., Butler S., Korpela H., Salonen R., Nyyssonen K., Palinski W., Witztum J.L. Autoantibody against oxidized LDL and progression of carotid atherosclerosis. Lancet. 339:1992;883-887.
Bui M.N., Sack M.N., Moutsatsos G., Lu D.Y., Katz P., McCown R., Breall J.A., Rackley C.E. Autoantibody titers to oxidized low-density lipoprotein in patients with coronary atherosclerosis. Am. Heart J. 131:1996;663-667.
Maggi E., Chiesa R., Melissano G., Castellano R., Astore D., Grossi A., Finardi G., Bellomo G. LDL oxidation in patients with severe carotid atherosclerosis. A study of in vitro and in vivo oxidation markers. Arterioscler. Thromb. 14:1994;1892-1899.
Maggi E., Marchesi E., Ravetta V., Martignoni A., Finardi G., Bellomo G. Presence of autoantibodies against oxidatively modified low-density lipoprotein in essential hypertension a biochemical signature of enhanced in vivo low-density lipoprotein. J. Hypertens. 13:1995;129-138.
Lehtimaki T., Lehtinen S., Solakivi T., Nikkila M., Jaakkola O., Jokela H., Yla-Herttuala S., Luoma J.S., Koivula T., Nikkari T. Autoantibodies against oxidized low density lipoprotein in patients with angiographically verified coronary artery disease. Arterioscler. Thromb. Vasc. Biol. 19:1990;23-27.
Omaye S.T., Turnbull J.D., Sauerlich H.E. Selected methods for the determination of ascorbic in animal cells, tissues, and fluids. Methods Enzymol. 62:1979;3-11.
Bieri J.G., Tolliver T.J., Catignani G.L. Simultaneous determination of α-tocopherol and retinol in plasma or red cells by high pressure liquid chromatography. Am. J. Clin. Nutr. 32:1979;2143-2149.
Nève, J.; Chamart, S.; Molle, L. Optimization of a direct procedure of selenium in plasma and erythrocytes using Zeeman effect atomic absorption spectrometry. In: Bratter, P.; Schamer, P., eds. Trace element analytical chemistry in medicine and biology. Berlin: Walter de Gruyter; 1987:1-10.
Tatzber, F.; Esterbauer, H. Autoantibodies to oxidized low-density lipoprotein. In: Rice-Evans, C.; Bellomo, G., eds. Free radicals IX. London: Richelieu Press; 1995: 245-262.
Maggi E., Finardi G., Poli M., Bollati P., Filipponi M., Stefano P.L., Paolini G., Grossi A., Clot P., Albano E., Bellomo G. Specificity of autoantibodies against oxidized LDL as an additional marker for atherosclerotic risk. Coron. Art. Dis. 4:1993;1119-1122.
Tatzber, F.; Lapin, A.; Temmel, C.; Wonish, W. Antibodies against oxidized LDL (olab) in Viennese working people. Abstract n°2 of Oxidative Stress Workshop of European Region Austrian Biochemical Study: Molecular Mechanisms and Pathophysiological Consequences of Oxidative Damage in Membranes and Lipoproteins. Seggau, Austria, 1995.
Meraji S., Ziouzenkova O., Resch U., Khoschsorur A., Tatzber F., Esterbauer H. Enhanced plasma level of lipid peroxidation in Iranians could be improved by antioxidants supplementation. Eur. J. Clin. Nutr. 51:1997;318-325.
Sanchez-Quesada, J. L.; Homs-Serradasanferm, R.; Serrat-Serrat, J.; Serra-Grima, J. R.; Gonzalez-Sastre, F.; Ordonez-Llanos, J. Increase of LDL susceptibility to oxidation occurring after intense, long-duration aerobic exercise. Atheroscler. 118:297-305; 1995.
Ahotupa, M.; Marniemi, J.; Lehtimäki, T.; Talvinen, K.; Raitakari, O. T.; Vasankari, T.; Viikari, J.; Luoma, J.; Ylä-Herttuala, S. Baseline diene conjugation in LDL lipids as a direct measure of in vivo LDL oxidation. Clin. Biochem. 31:257-261; 1998.
Vasankari T.J., Kujala U.M., Vasankari T.M., Vuorimaa T., Ahotupa M. Effects of acute prolonged exercise on serum and LDL oxidation and antioxidant defences. Free Radic. Biol. Med. 22:1997;509-513.
Mena P., Maynar M., Gutierrez J.M., Maynar J., Timon J., Campillo J.E. Erythrocyte free radical scavenger enzymes in bicycle professional racers. Adaptation to training. Int. J. Sports Med. 12:1991;563-566.
Hubner-Wozniak E., Lutoslawska G., Sendecki W., Sitkowski D., Borkowski L. Resting glutathione peroxidase activity in whole blood in response to various modes of training. Biol. Sport. 3:1996;267-272.
Brites F.D., Evelson P.A., Christiansen M.G., Nicol M.F., Basilico M.J., Wikinski R.W., Llesuy S.F. Soccer players under regular training show oxidative stress but an improved plasma antioxidant status. Clin. Sci. 96:1999;381-385.
Chiesa R., Melissano G., Castellano R., Astore D., Marone E.M., Grossi A., Maggi E., Finardi G., Casasco A., Bellomo G. In search of biological markers of high-risk carotid artery atherosclerotic plaque: Enhanced LDL oxidation. Ann. Vasc. Surg. 12:1998;1-9.