Reference : Potentiality of using microbial biosensors for the detection of substrate heterogenei...
Scientific congresses and symposiums : Paper published in a journal
Life sciences : Biotechnology
http://hdl.handle.net/2268/112130
Potentiality of using microbial biosensors for the detection of substrate heterogeneities and the assessment of microbial viability in industrial bioreactors: a complete set of experiments in chemostat and scale-down reactors, and elaboration of a mini scale-down platform
English
Brognaux, Alison [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
Neubauer, Peter [ > > ]
Twizere, Jean-Claude mailto [Université de Liège - ULg > Chimie et bio-industries > Biologie cell. et moléc. >]
Thonart, Philippe mailto [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
Delvigne, Frank mailto [Université de Liège - ULg > Chimie et bio-industries > Bio-industries >]
2012
Communications in Agricultural and Applied Biological Sciences
Universiteit Gent. Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen
77
1
3-7
Yes (verified by ORBi)
Yes
1379-1176
Gent
Belgium
17th Symposium on Applied and Biological Sciences
10 février 2012
Universiteit Gent. Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen
Leuven
Belgique
[en] biosensors ; scale-up ; stress gene
[en] Substrate limitation responsive biosensors have been used in order to detect spatial substrate heterogeneities, , inside industrial bioreactors (whole-cell biosensor). Three green fluorescent protein (GFP) transcriptional reporters have been chosen in E.coli, i.e. uspA::gfp, csiE::gfp and yciG::gfp. The promoter uspA is induced in response to a variety of stresses whereas the two other promoters, csiE and yciG, are supposed to be more specific in front of a substrate limitation.

The responsiveness of these biosensors has been assessed in chemostat reactor. Secondly, the same biosensors have been tested in well-mixed laboratory reactors and in scale-down reactors able to reproduce industrial conditions. Finally, a mini scale-down platform has been proposed as a high throughput tool to investigate rapidly the usefulness of a given microbial biosensor. Local heterogeneities in mini-bioreactor have caused a decrease of GFP expression, as in scale-down reactor. The presence of GFP in supernatants was noticed and this leakage seems to be correlated with the membrane permeability.
Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA
Researchers ; Students
http://hdl.handle.net/2268/112130

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