[en] Two recombinant DNA plasmids were constructed with identical transcriptional and translational regulatory elements controlling expression of the bovine growth hormone (bGH) gene or the bGH gene lacking introns. Transient expression of these plasmids in cultured eukaryotic cells, monitored by assaying secretion of bGH into the culture medium, was employed to examine the relative importance of introns in the expression of this gene. The bGH gene lacking introns is expressed more efficiently than the bGH gene in avian and mammalian cells.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Pasleau, Françoise ; Université de Liège - ULiège > CARE "Le Réseau des bibliothèques" > Bibliothèque des Sciences de la vie
Leung, F. C.
Kopchick, J. J.
Language :
English
Title :
A comparison of bovine growth hormone expression directed by bGH genomic or intronless DNA in transiently transfected eukaryotic cells.
Banerji, Oison, Schaffner (1983) A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes. Cell 33:729-740.
Birnbaum, Baxter (1986) Glucocorticoids regulate the expression of a rat growth hormone gene lacking 5' flanking sequences. J. Biol. Chem. 261:291-297.
de Villiers, Olson, Banerji, Schaffner (1982) Transcription ‘enhancers’ from SV40 and polyoma virus show a cell type preference. Nucleic Acids Research 10:7965-7976.
Gillies, Morrison, Oi, Tonegawa (1983) A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene. Cell 33:717-728.
Kopchick, Stacey (1984) Differences in intracellular DNA ligation after microinjection and transfection. Mol. Cell Biol. 4:240-246.
Kopchick, Malavarca, Livelli, Leung (1985) Use of avian retroviral-bovine growth hormone DNA recombinants to direct expression of biologically active growth hormone by cultured fibroblasts. DNA 4:23-31.
Kopchick, Pasleau, Leung (1986) Expression of the bovine growth hormone gene in cultured rodent cells, in genetic engineering of animals. Proceedings of the International Conference on Genetic Engineering of Animals: An Agricultural Perspective , W. Evans, A. Hollaender, University of California. Plenum Press, New York; 19-37.
Kostyo, Isaksson (1977) Growth hormone and the regulation of somatic growth. International Review of Physiology. Reproductive Physiology II , R.O. Greep, University Park Press, Baltimore, MD; 13:255-274.
Leung, Taylor, Steelman, Bennett, Rodkey, Long, Serio, Weppelman, Olson (1984) Purification and properties of chicken growth hormone and the development of a homologous radioimmunoassay. Gen. Comp. Endocrinol. 56:389-400.
Laimins, Tsichlis, Khoury (1984) Multiple enhancer domains in the 3' terminus of the Prague strain of Rous Sarcoma virus. Nucl. Acids Res. 12:6427-6442.
Lopata, Cleveland, Sollner-Webb (1984) High level transient expression of chloramphenicol acetyl transferase gene by DEAE-dextran mediated DNA transfection coupled with a dimethyl sulfoxide or glycerol shock treatment. Nucl. Acids Res. 12:5707-5717.
Luciw, Bishop, Varmus, Capecchi (1983) Location and function of retroviral and SV40 sequences that enhance biochemical transformation after microinjection of DNA. Cell 33:705-716.
Martin (1978) Neural regulation of growth hormone secretion. New England Journal of Medicine 288:1384-1388.
Miller, Martial, Baxter (1980) Molecular cloning of DNA complementary to bovine growth hormone mRNA. J. Biol. Chem. 255:7521-7524.
Moore, Marks, Buckley, Kapler, Payvar, Goodman (1985) The first intron of the human growth hormone gene contains a binding site for glucocorticoid receptor. Proc. Natl. Acad. Sci. USA 82:699-702.
Morikawa, Nixon, Green (1982) Growth hormone and the adipose conversion of 3T3 cells. Cell 29:783-789.
Morikawa, Green, Lewis (1984) Activity of human growth hormone and related polypeptides on the adipose conversion of 3T3 cells. Mol. Cell. Biol. 4:228-231.
Neuhaus, Neuhaus-Url, Gruss, Schweiger (1984) Enhancer-controlled expression of the simian virus 40 T antigen in the green alga acetaboparia. EMBO J. 3:2169-2172.
Palmiter, Brinster, Hammer, Taumbauer, Rosenfeld, Birnberg, Evans (1982) Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone fusion genes. Nature 300:611-615.
Pasleau, Tocci, Leung, Kopchick (1985) Growth hormone gene expression in eukaryotic cells directed by the Rous Sarcoma virus long terminal repeat or cytomegalovirus immediate-early promoter. Gene 38:227-232.
Pfaar, Sathe, Reff (1985) A highly modular cloning vector for the analysis of eukaryotic genes and gene regulatory elements. DNA 4:461-467.
Pfaar, Rieser, Woychik, Grottman, Rosenberg, Reff (1986) Differential effects of polyadenylation regions on gene expression in mammalian cells. DNA 5:115-122.
Picard, Schaffner (1984) A lymphocyte-specific enhancer in the mouse immunoglobulin k gene. Nature 307:80-82.
Roberts, Axel (1982) Gene amplification and gene correction in somatic cells. Cell 29:109-119.
Selden, Howie, Rowe, Goodman, Moore (1986) Human growth hormone as a reporter gene in regulation studies employing transient gene expression. Mol. Cell. Biol. 6:3173-3179.
Slater, Rabenau, Karin, Baxter, Beato (1985) Glucocorticoid receptor binding and activation of a heterologous promoter by dexamethasone by the first intron of the human growth hormone gene. Mol. Cell. Biol. 5:2984-2992.
Wigler, Silverstein, Lee, Pellicer, Cheng, Axel (1977) Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells. Cell 11:223-232.
Woychik, Camper, Lyons, Horowitz, Goodwin, Rottman (1982) Cloning and nucleotide sequencing of the bovine growth hormone gene. Nucl. Acids Res. 10:7197-7210.
Woychik, Lyons, Post, Rottman (1984) Requirements for the 3' flanking region of the bovine growth hormone gene for accurate polyadenylation. Proc. Natl. Acad. Sci. USA 81:3944-3948.
