Reference : Expression of a synthetic gene encoding human insulin-like growth factor I in cultured m...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/111880
Expression of a synthetic gene encoding human insulin-like growth factor I in cultured mouse fibroblasts.
English
Bayne, M. L. [ > > ]
Cascieri, M. A. [ > > ]
Kelder, B. [ > > ]
Appelbaum, J. [ > > ]
Chicchi, G. [ > > ]
Shapiro, J. A. [ > > ]
Pasleau, Françoise mailto [Université de Liège - ULg > CARE "Le Réseau des bibliothèques de l'ULg" > Bibliothèque des Sciences de la vie >]
Kopchick, J. J. [ > > ]
May-1987
Proceedings of the National Academy of Sciences of the United States of America
National Academy of Sciences
84
2638-2642
International
0027-8424
1091-6490
Washington
DC
[en] A synthetic gene encoding human insulin-like growth factor I (hIGF-I) was assembled and inserted into an expression vector containing the cytomegalovirus immediate early (CMV-IE) transcriptional regulatory region and portions of the bovine growth hormone gene. The recombinant plasmid encodes a 97 amino acid fusion protein containing the first 27 amino acids of the bovine growth hormone precursor and the 70 amino acids of hIGF-I. This plasmid, when transiently introduced into cultured mouse fibroblasts, directs synthesis of the fusion protein, subsequent proteolytic removal of the bovine growth hormone signal peptide, and secretion of hIGF-I into the culture medium. Conditioned medium from transfected cells inhibits binding of 125I-labeled IGF-I to type I IGF receptors on human placental membranes and to acid-stable human serum carrier proteins. The recombinant hIGF-I produced is biologically active, as monitored by the stimulation of DNA synthesis in vascular smooth muscle cells.
http://hdl.handle.net/2268/111880

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