Reference : Genome comparison of Bifidobacterium longum strains NCC2705 and CRC-002 using suppressio...
Scientific journals : Article
Life sciences : Microbiology
http://hdl.handle.net/2268/111747
Genome comparison of Bifidobacterium longum strains NCC2705 and CRC-002 using suppression subtractive hybridization.
English
Delcenserie, Véronique mailto [Université Laval > Département de sciences des aliments et de nutrition > Institut des Nutraceutiques et des Aliments Fonctionnels > 2007 >]
Lessard, Marie*-Helene [Université Laval > > >Département des sciences des aliments et de nutrition > > >]
LaPointe, Gisele [Université Laval > Département des sciences des aliments et de nutrition > > >]
Roy, Denis [Université Laval > Département des sciences des aliments et de nutrition > > >]
2008
FEMS Microbiology Letters
Blackwell Publishing
280
1
50-6
Yes (verified by ORBi)
International
0378-1097
1574-6968
Oxford
United Kingdom
[en] Adenosine Triphosphatases/genetics ; Bifidobacterium/classification/genetics ; Carbohydrates/genetics ; DNA, Bacterial/genetics ; Genome, Bacterial ; Genomic Library ; Nucleic Acid Hybridization/methods ; Peptide Hydrolases/genetics ; Polymerase Chain Reaction ; Polysaccharides/genetics/metabolism ; Sequence Analysis, DNA ; Species Specificity
[en] Because probiotic effects are strain dependent, genomic explanations of these differences will contribute to understanding their mechanisms of action. The genomic sequence of the Bifidobacterium longum probiotic strain NCC2705 was determined, but little is known about the genetic diversity between strains of this species. Suppression subtractive hybridization (SSH) is a powerful method for generating a set of DNA fragments differing between two closely related bacterial strains. The purpose of this study was to identify genetic differences between genomes of B. longum strains NCC2705 and CRC-002 using PCR-based SSH. Strain CRC-002 produces exopolysaccharides whereas NCC2705 is not known for reliable exopolysaccharide production. Thirty-five and 30 different sequences were obtained from the SSH libraries of strains CRC-002 and NCC2705, respectively. Specific CRC-002 genes found were predicted to be involved in the biosynthesis of exopolysaccharides and metabolism of other carbohydrates, and these genes were not present in the genome of strain NCC2705. The identification of an endo-1,4-beta-xylanase gene in the CRC-002 SSH library is an important difference because xylanase genes have previously been proposed as a defining characteristic of the NCC2705 strain. The results demonstrate that the SSH technique was useful to highlight potential genes involved in complex sugar metabolism that differ between the two probiotic strains.
http://hdl.handle.net/2268/111747
10.1111/j.1574-6968.2007.01037.x

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