ORBi: Temma-Asano K. - Effects of 4-Hydroxy-2-Nonenal, a Major Lipid Peroxidation-Derived Aldehyde, and N-Acetylcysteine on the Cyclooxygenase-2 Expression in Human Uterine Myometrium.

Reference : Effects of 4-Hydroxy-2-Nonenal, a Major Lipid Peroxidation-Derived Aldehyde, and N-Acety...


	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Reproductive medicine (gynecology, andrology, obstetrics)
	To cite this reference:	http://hdl.handle.net/2268/111671

Title :	Effects of 4-Hydroxy-2-Nonenal, a Major Lipid Peroxidation-Derived Aldehyde, and N-Acetylcysteine on the Cyclooxygenase-2 Expression in Human Uterine Myometrium.
Language :	English
Author, co-author :	Temma-Asano, K. [> > > >]
	Tskitishvili, Ekaterine [Université de Liège - ULg > Département des sciences cliniques > Labo de biologie des tumeurs et du développement >]
	Kanagawa, T. [> > > >]
	Tomimatsu, T. [> > > >]
	Tsutsui, T. [> > > >]
	Kimura, T. [> > > >]
	Chang, Y. S. [> > > >]
	Nakamura, T. [> > > >]
	Nakai, Y. [> > > >]
	Shimoya, K. [> > > >]
Publication date :	2011
Journal title :	Gynecologic & Obstetric Investigation
Publisher :	S. Karger
Peer reviewed :	Yes (verified by ORBi)
Audience :	International
ISSN :	0378-7346
e-ISSN :	1423-002X
City :	Basel
Country :	Switzerland
Keywords :	[en] Prostaglandin,Preterm labor,Oxidative stress ; Myometrium, N-Acetylcysteine ; 4-Hydroxy-2-nonenal, Cyclooxygenase-2
Abstract :	[en] Background: Chorioamnionitis is one of the important causes of preterm labor. Preterm labor with chorioamnionitis is associated with oxidative stress. We reported that 4-hydroxy-2-nonenal (4-HNE), a major end product of oxidative fatty acid metabolism, is accumulated in the placenta with chorioamnionitis. The aim of this study was to confirm the effect of 4-HNE on cyclooxygenase-2 (COX-2) and prostaglandin (PG) induction in the uterine myometrial tissues. We also examined the effect of N-acetylcysteine (NAC) on 4-HNE-induced COX-2 expression. Methods: Uterine myometrial tissues were obtained from 5 patients. One of them underwent elective cesarean section without labor, and 4 of them underwent hysterectomy because of placental previa or atonic bleeding. We stimulated the uterine myometrial tissues with 4-HNE. In addition, the tissues were pretreated with NAC before 4-HNE treatment. The expression of COX-2 mRNA was observed by real-time PCR. PGE2 and prostacyclin release into the supernatants of the tissue cultures was measured by ELISA. Results: 4-HNE induced the COX-2 mRNA expression and PGE2 production in the uterine myometrial tissue culture in a dose-dependent and time-dependent manner. NAC inhibited 4-HNE-induced COX-2 expression. Conclusion: 4-HNE may play an important role in preterm labor. NAC might be protective against preterm labor under oxidative stress.
Target :	Researchers ; Professionals ; Students
Permalink :	http://hdl.handle.net/2268/111671
DOI :	10.1159/000322393
Commentary :	Copyright (c) 2011 S. Karger AG, Basel.

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Restricted access	Effects of 4-Hydroxy-2-Nonenal, a Major Lipid Peroxidation-Derived Aldehyde, and N-Acetylcysteine on the Cyclooxygenase-2 Expression in Human Uterine Myometrium.docx	This is the Author’s Preprint of an article published in Gynecological and Obstetric Investigation; 2011; 72(1):37-42. The original published version is available at: http://content.karger.com/produktedb/produkte.asp?DOI=000322393&typ=pdf	Author preprint	84.53 kB	Request copy

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