Reference : Processing samples of benthic marine diatoms from Mediterranean oligotrophic areas
Scientific journals : Article
Life sciences : Aquatic sciences & oceanology
Life sciences : Environmental sciences & ecology
Life sciences : Phytobiology (plant sciences, forestry, mycology...)
http://hdl.handle.net/2268/111509
Processing samples of benthic marine diatoms from Mediterranean oligotrophic areas
English
Vermeulen, Simon mailto [Université de Liège - ULg > > > Doct. sc. (océanogr. - Bologne)]
Lepoint, Gilles mailto [Université de Liège - ULg > Département de Biologie, Ecologie et Evolution > Océanologie >]
Gobert, Sylvie mailto [Université de Liège - ULg > Département de Biologie, Ecologie et Evolution > Océanologie >]
2012
Journal of Applied Phycology
Springer
24
1253-1260
Yes (verified by ORBi)
International
0921-8971
1573-5176
Dordrecht
Netherlands
[en] Bacillariophyta ; Methods ; Cell losses ; Cleaning techniques ; Silicification
[en] The processing of benthic diatoms is tedious and involves several potentially damaging steps for cells. Although the preservation of siliceous frustules is of paramount importance in the implementation of biotic indices, only few studies quantified treatment-induced cell losses. We assumed that commonly used treatments may lead to mechanical (centrifugation, sedimentation, boiling, sonication and mounting in Naphrax) and chemical (cold H2O2 digestion) damages on diatoms. We analysed the potential adverse effects of these treatments and the cleaning efficiency of H2O2 and incineration in order to find out the most suitable technique to process lightly silicified Mediterranean populations. Results showed that successive resuspensions of material after each concentration treatment (sedimentation and centrifugation) and low speed centrifugation did not alter the physical integrity of frustules. In contrast, boiling and sonication exhibited adverse effects especially on the preservation of large frustules and Naphrax mounting proved to be the most damaging step whatever the size of diatoms. For cleaning treatments, incineration provided the most satisfactory results and acted on a non-selective way as opposed to hydrogen peroxide which led to either a large number of non-cleaned frustules or dissolved valves. Our recommendations for processing samples of lightly silicified Mediterranean benthic diatoms include the use of low speed centrifugations, dehydration at room temperature, incineration and dry mounting.
Centre Interfacultaire de Recherches en Océanologie - MARE
Belgian National Fund for Scientific Research ; French Belgian community
ARC - RACE 05/10-333
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/111509
10.1007/s10811-011-9770-4
The final publication is available at www.springerlink.com

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Open access
Author_post_review_Vermeulen2011_JAPH.pdfAuthor postprint374.95 kBView/Open

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.