Reference : Studying RNA-Protein Interactions In Vivo By RNA Immunoprecipitation
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/111374
Studying RNA-Protein Interactions In Vivo By RNA Immunoprecipitation
English
Selth, L. A. [ > > ]
Close, Pierre mailto [Université de Liège - ULg > Département de pharmacie > Chimie médicale >]
Svejstrup, J. Q. [ > > ]
2011
Methods in Molecular Biology (Clifton, N.J.)
Humana Press
791
253-264
Yes (verified by ORBi)
International
1064-3745
Totowa
NJ
[en] RNA immunoprécipitation ; transcription ; RNA binding protein
[en] The crucial roles played by RNA-binding proteins in all aspects of RNA metabolism, particularly in the regulation of transcription, have become increasingly evident. Moreover, other factors that do not directly interact with RNA molecules can nevertheless function proximally to RNA polymerases and have significant effects on gene expression. RNA immunoprecipitation (RIP) is a powerful technique used to detect direct and indirect interactions between individual proteins and specific RNA molecules in vivo. Here, we describe RIP methods for both yeast and mammalian cells.
http://hdl.handle.net/2268/111374

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