Reference : The structure at 2 A resolution of Phycocyanin from Gracilaria chilensis and the energy ...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/111234
The structure at 2 A resolution of Phycocyanin from Gracilaria chilensis and the energy transfer network in a PC-PC complex.
English
Contreras-Martel, Carlos [> > > >]
Matamala, Adelio [> > > >]
Bruna, Carola [> > > >]
Poo-Caamano, German [> > > >]
Almonacid, Daniel [> > > >]
Figueroa, Maximiliano mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Martinez-Oyanedel, Jose [> > > >]
Bunster, Marta [> > > >]
2007
Biophysical Chemistry
Elsevier Science
125
2-3
388-96
Yes (verified by ORBi)
0301-4622
Amsterdam
The Netherlands
[en] Crystallography, X-Ray ; Energy Transfer ; Gracilaria/chemistry ; Macromolecular Substances/chemistry ; Molecular Structure ; Phycocyanin/chemistry
[en] Phycocyanin is a phycobiliprotein involved in light harvesting and conduction of light to the reaction centers in cyanobacteria and red algae. The structure of C-phycocyanin from Gracilaria chilensis was solved by X-ray crystallography at 2.0 A resolution in space group P2(1). An interaction model between two PC heterohexamers was built, followed by molecular dynamic refinement. The best model showed an inter-hexamer rotation of 23 degrees . The coordinates of a PC heterohexamer (alphabeta)(6) and of the PC-PC complex were used to perform energy transfer calculations between chromophores pairs using the fluorescence resonance energy transfer approach (FRET). Two main intra PC ((I)beta(3)(82)-->(I)alpha(1)(84)-->(I)alpha(5)(84)-->(I)beta(6)(82) and (I)beta(3)(153)-->(I)beta(5)(153)) and two main inter PC ((I)beta(6)(82)-->(II)beta(3)(82) and (I)beta(5)(153)-->(II)beta(3)(153)) pathways were proposed based on the values of the energy transfer constants calculated for all the chromophore pairs in the hexamer and in the complex.
http://hdl.handle.net/2268/111234
10.1016/j.bpc.2006.09.014

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