ORBi: Beckers Jean-François - Bovine follitropin: Isolation and characterization of the native hormone and its alpha and ß subunits

Reference : Bovine follitropin: Isolation and characterization of the native hormone and its alpha a...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/1107

Title :	Bovine follitropin: Isolation and characterization of the native hormone and its alpha and ß subunits
Language :	English
Alternative title :	[fr] Follitropine bovine: Isolement et caractérisation de l'hormone native et de ses sous-unités
Author, co-author :	Beckers, Jean-François [Université de Liège - ULg - Faculté de Médecine Vétérinaire > Chaire d'Obstétrique et de Gynécologie > > >]
	Closset, Jean [Université de Liège - ULg - CHU > Section d'Endocrinologie > > >]
	Maghuin-Rogister, Guy [Université de Liège - ULg - CHU > Section d'Endocrinologie > > >]
	Hennen, Georges [Centre Hospitalier Universitaire de Liège - CHU > > Endocrinologie clinique > > > >]
Publication date :	1977
Journal title :	Biochimie
Publisher :	Elsevier
Volume :	59
Pages :	825-831
Audience :	International
ISSN :	0300-9084
City :	Paris
Country :	France
Keywords :	[en] Bovine follitropin ; Subunits alpha and beta ; Bovine
	[fr] Follitropine bovine ; Sous unités alpha et beta ; Bovin
Abstract :	[en] 1. A reproducible procedure was developed for the purification of bovine follitropin. 2. The methode involved ammonium sulfate precipitation, ion exchange and adsorption chromatography, concanavaline-A-Sepharose chromatography and gel filtration. 3. A specific radioligand receptor assay was used to monitor each chromatographical step. 4. The potency of highly purified bovine follitropin as measured by Steelman and Pohley bioassay was 62 times the NIH-FSH-B1 standard preparation. 5. Contaminations of bovine follitropin by other glycoprotein hormones such as thyrotropin and lutropin amounted to 3 and 0.45 per cent by weight respectively as measured by specific radioimmunoassays and radioligand receptor assays. 6. The subunits alpha and beta of bovine follitropin were obtained by incubation in acidic urea, the chains being then separated by anion exchange chromatography. The subunits were submitted to complete characterization. The amino terminal residue of the alpha subunit is phenylalanine while a half cystine residue was found at the amino-terminal end of the beta chain. 8. Cross-contamination of the alpha and beta subunit preparations was measured by specific radioimmunoassays and amounted to 0.02 and 0.1 per cent by weight respectively.
Research units :	Laboratoire d'Endocrinologie et de Reproduction Animale
Funders :	I.R.S.I.A.
Name of the research project :	Etude de la stérilité hormonale chez les bovins
Target :	Researchers ; Professionals ; Students
Permalink :	http://hdl.handle.net/2268/1107

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