|Reference : Bovine follitropin: Isolation and characterization of the native hormone and its alpha a...|
|Scientific journals : Article|
|Life sciences : Biochemistry, biophysics & molecular biology|
|Bovine follitropin: Isolation and characterization of the native hormone and its alpha and ß subunits|
|[fr] Follitropine bovine: Isolement et caractérisation de l'hormone native et de ses sous-unités|
|Beckers, Jean-François [Université de Liège - ULg - Faculté de Médecine Vétérinaire > Chaire d'Obstétrique et de Gynécologie > > >]|
|Closset, Jean [Université de Liège - ULg - CHU > Section d'Endocrinologie > > >]|
|Maghuin-Rogister, Guy [Université de Liège - ULg - CHU > Section d'Endocrinologie > > >]|
|Hennen, Georges [Centre Hospitalier Universitaire de Liège - CHU > > Endocrinologie clinique > > > >]|
|[en] Bovine follitropin ; Subunits alpha and beta ; Bovine|
|[fr] Follitropine bovine ; Sous unités alpha et beta ; Bovin|
|[en] 1. A reproducible procedure was developed for the purification of bovine follitropin.
2. The methode involved ammonium sulfate precipitation, ion exchange and adsorption chromatography, concanavaline-A-Sepharose chromatography and gel filtration.
3. A specific radioligand receptor assay was used to monitor each chromatographical step.
4. The potency of highly purified bovine follitropin as measured by Steelman and Pohley bioassay was 62 times the NIH-FSH-B1 standard preparation.
5. Contaminations of bovine follitropin by other glycoprotein hormones such as thyrotropin and lutropin amounted to 3 and 0.45 per cent by weight respectively as measured by specific radioimmunoassays and radioligand receptor assays.
6. The subunits alpha and beta of bovine follitropin were obtained by incubation in acidic urea, the chains being then separated by anion exchange chromatography. The subunits were submitted to complete characterization. The amino terminal residue of the alpha subunit is phenylalanine while a half cystine residue was found at the amino-terminal end of the beta chain.
8. Cross-contamination of the alpha and beta subunit preparations was measured by specific radioimmunoassays and amounted to 0.02 and 0.1 per cent by weight respectively.
|Laboratoire d'Endocrinologie et de Reproduction Animale|
|Etude de la stérilité hormonale chez les bovins|
|Researchers ; Professionals ; Students|
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