Reference : Disturbed Cytokine Production at the Systemic Level in Difficult-to-Control Atopic Asthm...
Scientific journals : Article
Human health sciences : Cardiovascular & respiratory systems
http://hdl.handle.net/2268/110658
Disturbed Cytokine Production at the Systemic Level in Difficult-to-Control Atopic Asthma: Evidence for Raised Interleukin-4 and Decreased Interferon-gamma Release following Lipopolysaccharide Stimulation.
English
MANISE, Maïté [Centre Hospitalier Universitaire de Liège - CHU > > Microbiologie médicale >]
SCHLEICH, FLorence [Centre Hospitalier Universitaire de Liège - CHU > > Pneumologie-Allergologie]
QUAEDVLIEG, Valérie [Centre Hospitalier Universitaire de Liège - CHU > > Pneumologie-Allergologie]
MOERMANS, Catherine [ > > ]
HENKET, Monique [Centre Hospitalier Universitaire de Liège - CHU > > Pneumologie-Allergologie]
SELE, Jocelyne [Centre Hospitalier Universitaire de Liège - CHU > > Pneumologie-Allergologie]
CORHAY, Jean-Louis [Centre Hospitalier Universitaire de Liège - CHU > > Pneumologie-Allergologie >]
LOUIS, Renaud mailto [Centre Hospitalier Universitaire de Liège - CHU > > Pneumologie-Allergologie]
2012
International Archives of Allergy & Immunology
S. Karger
158
1
1-8
Yes (verified by ORBi)
International
1018-2438
1423-0097
Basel
Switzerland
[en] Background: Disturbed cytokine production is thought to govern inflammation in asthma, which, in its turn, may lead to uncontrolled disease. The aim of this study was to assess the relationship between cytokine production from blood leucocytes and the level of asthma control. Methods: We compared the production of interleukin (IL)-4, IL-6, IL-10, interferon (IFN)-gamma and tumour necrosis factor-alpha from peripheral blood leucocytes in non-atopic healthy subjects (n = 22), atopic non-asthmatics (n = 10), well-controlled asthmatics [Juniper asthma control questionnaire (ACQ) score <1.5; n = 20] and patients with uncontrolled asthma despite inhaled or oral corticoids (ACQ score >/=1.5; n = 20). Fifty microlitres of peripheral blood was incubated for 24 h with RPMIc, lipopolysaccharide (LPS; 1 ng/ml) or phytohaemagglutinin (1 mug/ml), and cytokines were measured by immunotrapping (ELISA). Results: Both controlled and uncontrolled asthmatics as well as atopic non-asthmatics spontaneously produced more IL-4 than non-atopic healthy subjects (p < 0.001). IL-4 production induced by LPS was significantly greater (p < 0.05) in both asthma groups compared to atopic non-asthmatics and non-atopic healthy subjects. By contrast, IFN-gamma release induced by LPS was lower in uncontrolled asthmatics than in non-atopic healthy subjects (p < 0.05) and controlled asthmatics (p < 0.05). IL-10 release after LPS was greater in uncontrolled asthmatics than in atopic non-asthmatics (p < 0.05). No difference was observed regarding other cytokines. Conclusion: Blood cells from patients with difficult-to-control atopic asthma display highly skewed Th2 cytokine release following LPS stimulation.
http://hdl.handle.net/2268/110658
10.1159/000329858
Copyright (c) 2011 S. Karger AG, Basel.

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
Disturbed cytokine production at the systemic level in difficult to control atopic_Manise_12_IntArchAlllergy.pdfPublisher postprint221.6 kBRequest copy
Open access
Disturbed cytokine production at the systemic level in difficult to control atopic_Manise_12_IntArchAlllergy.pdfAuthor postprint100.69 kBView/Open

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.