Article (Scientific journals)
Reconstruction of a human mitochondrial complex I mutation in the unicellular green alga Chlamydomonas.
Larosa, Véronique; Coosemans, Nadine; Motte, Patrick et al.
2012In Plant Journal, 70, p. 759-768
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Abstract :
[en] Defects in complex I (NADH:ubiquinone oxidoreductase) are the most frequent cause of human respiratory disorders. The pathogenicity of a given human mitochondrial mutation can be difficult to demonstrate because the mitochondrial genome harbors large numbers of polymorphic base changes that have no pathogenic significance. In addition, mitochondrial mutations are usually found in the heteroplasmic state, which could hide the biochemical effect of the mutation. We propose that the unicellular green alga Chlamydomonas could be used to study such mutations because (1) respiratory-deficient mutants are viable and mitochondrial mutations are found in the homoplasmic state, (2) transformation of the mitochondrial genome is feasible, (3) Chlamydomonas complex I is close to that of humans. To illustrate that, we have introduced a Leu157Pro substitution in the Chlamydomonas ND4 subunit of complex I of two different recipient strains by biolistic transformation, demonstrating that site-directed mutagenesis of the Chlamydomonas mitochondrial genome is possible. This substitution did not lead to any respiratory enzyme defect when it is present in the heteroplasmic state in a patient presenting chronic progressive external ophthalmoplegia. When present in the homoplasmic state in the alga, the mutation does not prevent the assembly of the 950 kDa whole complex I which conserves nearly all the NADH dehydrogenase activity of the peripheral arm. However, the NADH:duroquinone oxidoreductase activity is strongly reduced, suggesting that the substitution could affect ubiquinone fixation to the membrane domain. The in vitro defects are correlated in vivo with a decrease in dark respiration and growth rate.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Larosa, Véronique ;  Université de Liège - ULiège > Département des sciences de la vie > Génétique
Coosemans, Nadine ;  Université de Liège - ULiège > Département des sciences de la vie > DES en biotechnologie
Motte, Patrick ;  Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale
Bonnefoy, Nathalie
Remacle, Claire  ;  Université de Liège - ULiège > Département des sciences de la vie > Génétique
Language :
English
Title :
Reconstruction of a human mitochondrial complex I mutation in the unicellular green alga Chlamydomonas.
Publication date :
January 2012
Journal title :
Plant Journal
ISSN :
0960-7412
eISSN :
1365-313X
Publisher :
Blackwell, Oxford, United Kingdom
Volume :
70
Pages :
759-768
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 30 January 2012

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