|Reference : Cytokine Modulation of Basophil Histamine Release in Wasp-Venom Allergy|
|Scientific journals : Article|
|Human health sciences : Cardiovascular & respiratory systems|
|Cytokine Modulation of Basophil Histamine Release in Wasp-Venom Allergy|
|Radermecker, Maurice [Université de Liège - ULg > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine) >]|
|Louis, Renaud [Université de Liège - ULg > Département des sciences cliniques > Pneumologie - Allergologie]|
|leclercq, M. [ > > ]|
|Weber, T. [> > > >]|
|Corhay, Jean-Louis [Université de Liège - ULg > > Pneumologie-Allergologie >]|
|Bury, Thierry [Université de Liège - ULg > Département des sciences de la motricité > Physiologie humaine et physiologie de l'effort physique]|
|Yes (verified by ORBi)|
|[en] We report the effect of interleukin-3 (IL-3) and of other cytokines on antigen-induced basophil histamine release in wasp-venom-allergic subjects. Leukocytes from 12 patients with documented anaphylactic sensitivity to wasp venom were preincubated in the presence or absence of IL-3, granulocyte/macrophage-colony stimulating factor (GM-CSF), IL-5, IL-8, or stem cell factor (SCF). Washed cells were then exposed to venom and to other secretagogues, and histamine release in the supernatant was measured fluorometrically. Preincubation of leukocytes with IL-3, GM-CSF, or IL-5 (0.02-2 ng/ml), but not with IL-8 and SCF, caused a dose-dependent enhancement of antigen-induced basophilic histamine release in all subjects tested. Mean maximum increase was about 100% for IL-3, IL-5, and GM-CSF. The priming effect of IL-3 was rapid, persisted up to 12 h, and was not accompanied by a change in cellular histamine. IL-3 had a comparable enhancing effect when basophils were triggered with anti-IgE or N-formylmethionylphenylalanine (FMP). By contrast, IL-3 had no effect on substance-P-induced histamine release. The significant enhancement of basophil releasability to antigen in wasp-venom allergy by very low concentrations of IL-3, GM-CSF, and IL-5 suggests that cytokines in the basophil (mast-cell?) microenvironment could be critical factors in determining the variability of sting reactions in Hymenoptera-venom-allergic subjects.|
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