Article (Scientific journals)
A23187 Ionophore—ca2+ interfacial complex: a monolayer approach
Ferreira, J.; Caspers, J.; Brasseur, Robert et al.
1981In Journal of Colloid and Interface Science, 81 (1), p. 158-168
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Abstract :
[en] This paper presents a new approach to study A23187 ionophore in an aqueous environment. The ionophore was spread at the air—water interface. Surface pressure and tritium—hydrogen surface exchange measurements demonstrated the conformational stability of A23187 in the free and complexed forms at the interface. Exchange measurements suggested that the A23187 ionophore adopts an interfacial conformation in which hydrogen bonds are not accessible to the aqueous solvent. Surface potential measurements allowed the calculation of the intrinsic dissociation constant of the ionophore carboxylic group in an aqueous environment (pKi = 5). The ionophore activity sequence for bivalent ions was Ca2+>Mg2+⇓Ba2+ This sequence is comparable to those obtained by other authors inorganic solution. Surface radioactivity measurements performed with 45Ca2+ in the subphase demonstrated the formation of a neutral complex between two negatively charged ionophore molecules and one Ca2+ ion. This reaction was only observed at pH higher than 5. The association constant of the interfacial ionophore-Ca2+ complex was calculated.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Ferreira, J.
Caspers, J.
Brasseur, Robert ;  Université de Liège - ULiège > Chimie et bio-industries > Centre de Bio. Fond. - Section de Biophysique moléc. numér.
Ruysschaert, J. M.
Language :
English
Title :
A23187 Ionophore—ca2+ interfacial complex: a monolayer approach
Publication date :
May 1981
Journal title :
Journal of Colloid and Interface Science
ISSN :
0021-9797
eISSN :
1095-7103
Publisher :
Academic Press, Orlando, United States - Florida
Special issue title :
Journal of Colloid and Interface Science
Volume :
81
Issue :
1
Pages :
158-168
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 20 January 2012

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