Reference : A peptide mimicking the C-terminal part of the reactive center loop induces the trans...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/108868
A peptide mimicking the C-terminal part of the reactive center loop induces the transition to the latent form of Plasminogen Activator Inhibitor Type-1
English
D'Amico, Salvino [Université de Liège - ULg > > GIGA-Research >]
Martial, Joseph mailto [Université de Liège - ULg > Département des sciences de la vie > Département des sciences de la vie >]
Struman, Ingrid mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
2012
FEBS Letters
Elsevier Science
586
686-692
Yes (verified by ORBi)
International
0014-5793
Amsterdam
The Netherlands
[en] Plasminogen activator inhibitor-1 ; Reactive Centre Loop ; peptide
[en] Plasminogen Activator Inhibitor-1 (PAI-1) is the primary inhibitor of plasminogen activators (uPA and tPA) and thus plays a central role in fibrinolysis. The spontaneous insertion of its Reactive Centre Loop (RCL) into beta-sheet A is responsible for its irreversible conversion into the inactive latent form. In this study, we used two peptides mimicking residues P14-P9 and P8-P3 of the RCL so as to understand this dynamic process. We show that both peptides inhibit the formation of PAI 1/uPA and PAI-1/tPA complexes via two different mechanisms. Targeting the N-terminal part of the loop induces the cleavage of PAI-1 by the proteases uPA/tPA while targeting its C-terminal part greatly favors the irreversible formation of latent PAI-1.
http://hdl.handle.net/2268/108868

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