ORBi: Cassart Dominique - Morphological alterations in oxidative muscles and mitochondrial structure associated with equine atypical myopathy

Reference : Morphological alterations in oxidative muscles and mitochondrial structure associated wi...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Veterinary medicine & animal health
	To cite this reference:	http://hdl.handle.net/2268/108785

Title :	Morphological alterations in oxidative muscles and mitochondrial structure associated with equine atypical myopathy
Language :	English
Author, co-author :	Cassart, Dominique [Université de Liège - ULg > Département de morphologie et pathologie > Département de morphologie et pathologie >]
	Baise, Etienne [Université de Liège - ULg > Département des sciences de la vie > Macromolécules biologiques >]
	Cherel, Yves [ > > ]
	Delguste, Catherine [Université de Liège - ULg > Services généraux (Faculté de médecine vétérinaire) > Scientifiques attachés au Doyen (F MV) >]
	Antoine, Nadine [Université de Liège - ULg > Département de morphologie et pathologie > Histologie >]
	Votion, Dominique [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
	Amory, Hélène [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Médecine interne des équidés >]
	Rollin, Frédéric [Université de Liège - ULg > Département clinique des animaux de production (DCP) > Médecine interne des équidés, des ruminants et des porcs >]
	Linden, Annick [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Santé et pathologies de la faune sauvage >]
	Coignoul, Freddy [Université de Liège - ULg > Services administratifs généraux > Vice-Recteur à la Qualité >]
	Desmecht, Daniel [Université de Liège - ULg > Département de morphologie et pathologie > Pathologie spéciale et autopsies >]
Publication date :	Jan-2007
Journal title :	Equine Veterinary Journal
Publisher :	Equine Veterinary Journal Ltd
Volume :	39
Issue/season :	1
Pages :	26-32
Audience :	International
ISSN :	0425-1644
City :	Newmarket Suffolk
Country :	United Kingdom
Keywords :	[en] myopathy ; horse ; mitochondria
Abstract :	[en] REASONS FOR PERFORMING STUDY: There is a lack of well documented studies about muscular lesions in equine atypical myopathy (EAM). OBJECTIVES: To characterise morphopathological changes of striated muscles and myocardium, to progress understanding of this disease. METHODS: Thirty-two horses age 0.5-7 years kept on pasture were referred for a sudden ataxia/myoglobinuria syndrome. Clinical examination (stiffness, muscle pain, muscle fasciculations, abnormal gait, recumbency, myoglobinuria, tachycardia, sweating) and plasma CPK, LDH and AST levels were consistent with extensive myonecrosis and, together with anamnestic data, with so-called 'equine atypical myopathy' (EAM), a disease of unknown aetiology reported since 1939. Macroscopic and microscopic (histology, histoenzymology, ultrastructure) lesions were evaluated. RESULTS: Necropsic examination revealed large areas of muscle necrosis, the extent and severity of which varied between cases and muscles, but which were clearly more constant and severe in respiratory and postural muscles and in the myocardium. Histology highlighted a multifocal and monophasic process compatible with Zenker degeneration/necrosis that mostly and segmentally affected type 1 fibres. Histochemical evaluation revealed a weak and disorganised pattern of NADH tetrazolium reductase staining, the absence of calcium salts precipitates and a dramatic accumulation of lipid droplets. Ultrastructural examination often revealed fibres of which the sole modifications were altered mitochondria and sarcoplasmic lipidosis. CONCLUSIONS: Taken together, the data suggest that a primary alteration of mitochondria should be considered, although secondary mitochondrial abnormalities have yet to be ruled out. POTENTIAL RELEVANCE: The morphological features gathered here reveal that EAM shares most of the characteristics of toxic myopathies.
Target :	Researchers ; Professionals
Permalink :	http://hdl.handle.net/2268/108785

File(s) associated to this reference

Fulltext file(s):

	File	Commentary	Version	Size	Access
Restricted access	EVJ 06-062 Cassart.pdf		Publisher postprint	578.99 kB	Request copy

All documents in ORBi are protected by a user license.

Dspace v1.5