Article (Scientific journals)
Discovery and biochemical characterisation of four novel biomarkers for osteoarthritis.
DE SENY, Dominique; Sharif, Mohammed; Fillet, Marianne et al.
2011In Annals of the Rheumatic Diseases, 70 (6), p. 1144-52
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Keywords :
Adult; Aged; Aged, 80 and over; Arthritis, Rheumatoid/blood/diagnosis; Biological Markers/analysis/blood; Blood Proteins/analysis; Case-Control Studies; Diagnosis, Differential; Female; Humans; Male; Middle Aged; Osteoarthritis, Knee/blood/diagnosis/metabolism; Peptide Fragments/analysis/blood; Proteomics/methods; Reproducibility of Results; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods; Synovial Fluid/chemistry
Abstract :
[en] OBJECTIVE: Knee osteoarthritis (OA) is a heterogeneous, complex joint pathology of unknown aetiology. Biomarkers have been widely used to investigate OA but currently available biomarkers lack specificity and sensitivity. Therefore, novel biomarkers are needed to better understand the pathophysiological processes of OA initiation and progression. METHODS: Surface enhanced laser desorption/ionisation-time of flight-mass spectrometry proteomic technique was used to analyse protein expression levels in 284 serum samples from patients with knee OA classified according to Kellgren and Lawrence (K&L) score (0-4). OA serum samples were also compared to serum samples provided by healthy individuals (negative control subjects; NC; n=36) and rheumatoid arthritis (RA) patients (n=25). Proteins that gave similar signal in all K&L groups of OA patients were ignored, whereas proteins with increased or decreased levels of expression were selected for further studies. RESULTS: Two proteins were found to be expressed at higher levels in sera of OA patients at all four K&L scores compared to NC and RA, and were identified as V65 vitronectin fragment and C3fpeptide. Of the two remaining proteins, one showed increased expression (unknown protein at m/z of 3762) and the other (identified as connective tissue-activating peptide III protein) was decreased in K&L scores >2 subsets compared to NC, RA and K&L scores 0 or 1 subsets. CONCLUSION: The authors detected four unexpected biomarkers (V65 vitronectin fragment, C3f peptide, CTAP-III and m/z 3762 protein) that could be relevant in the pathophysiological process of OA as having significant correlation with parameters reflecting local inflammation and bone remodelling, as well as decrease in cartilage turnover.
Disciplines :
Gastroenterology & hepatology
Author, co-author :
DE SENY, Dominique ;  Centre Hospitalier Universitaire de Liège - CHU > Rhumatologie
Sharif, Mohammed
Fillet, Marianne ;  Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
COBRAIVILLE, Gaël ;  Centre Hospitalier Universitaire de Liège - CHU > Rhumatologie
Meuwis, Marie-Alice  ;  Centre Hospitalier Universitaire de Liège - CHU > Gastro-Entérologie-Hépatologie
Marée, Raphaël  ;  Université de Liège - ULiège > GIGA-Management : Plateforme bioinformatique
HAUZEUR, Jean-Philippe ;  Centre Hospitalier Universitaire de Liège - CHU > Rhumatologie
Wehenkel, Louis  ;  Université de Liège - ULiège > Dép. d'électric., électron. et informat. (Inst.Montefiore) > Systèmes et modélisation
Louis, Edouard  ;  Centre Hospitalier Universitaire de Liège - CHU > Gastro-Entérologie-Hépatologie
Merville, Marie-Paule ;  Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Kirwan, John
Ribbens, Clio ;  Université de Liège - ULiège > Département des sciences cliniques > Rhumatologie
Malaise, Michel ;  Université de Liège - ULiège > Département des sciences cliniques > Rhumatologie
More authors (3 more) Less
Language :
English
Title :
Discovery and biochemical characterisation of four novel biomarkers for osteoarthritis.
Publication date :
2011
Journal title :
Annals of the Rheumatic Diseases
ISSN :
0003-4967
eISSN :
1468-2060
Publisher :
BMJ Group, London, United Kingdom
Volume :
70
Issue :
6
Pages :
1144-52
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 16 January 2012

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