Reference : Optimization of micro-HPLC peak focusing for the detection and quantification of low hep...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
http://hdl.handle.net/2268/107676
Optimization of micro-HPLC peak focusing for the detection and quantification of low hepcidin concentrations.
English
Mansion, François mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments]
Chiap, Patrice mailto [Université de Liège - ULg > Département de pharmacie > Département de pharmacie]
Houbart, Virginie mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Crommen, Jacques mailto [Université de Liège - ULg > Département de pharmacie > Département de pharmacie]
Servais, Anne-Catherine mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments]
Fillet, Marianne mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments]
Jul-2011
Journal of Separation Science
Wiley-VCH Verlag Gmbh
34
15
1820-1827
Yes (verified by ORBi)
International
1615-9306
1615-9314
Weinheim
Germany
[en] Micro-high-performance liquid chromatography is a miniaturized, economic and ecological chromatographic system allowing the use of reduced size chromatographic columns. Coupled with electrospray ionization tandem mass spectrometry, this technique can be used to detect and quantify low concentrations of peptides. In this study, hepcidin was used as the model compound and analysed using octadecylsilica stationary phase by means of a gradient elution mode at a flow rate of 4 muL/min. Several parameters were studied to optimize peak focusing. Using the methodology of experimental design, the mobile-phase gradient conditions and the sample composition were optimized in order to maximize the sensitivity and minimize retention time. Stability of the target peptide in solution was also demonstrated.
Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA ; Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS
http://hdl.handle.net/2268/107676
10.1002/jssc.201100244
Copyright (c) 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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