[en] Micro-high-performance liquid chromatography is a miniaturized, economic and ecological chromatographic system allowing the use of reduced size chromatographic columns. Coupled with electrospray ionization tandem mass spectrometry, this technique can be used to detect and quantify low concentrations of peptides. In this study, hepcidin was used as the model compound and analysed using octadecylsilica stationary phase by means of a gradient elution mode at a flow rate of 4 muL/min. Several parameters were studied to optimize peak focusing. Using the methodology of experimental design, the mobile-phase gradient conditions and the sample composition were optimized in order to maximize the sensitivity and minimize retention time. Stability of the target peptide in solution was also demonstrated.
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Mansion, François ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Chiap, Patrice ; Université de Liège - ULiège > Département de pharmacie > Département de pharmacie
Houbart, Virginie ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Crommen, Jacques ; Université de Liège - ULiège > Département de pharmacie > Département de pharmacie
Servais, Anne-Catherine ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Fillet, Marianne ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Language :
English
Title :
Optimization of micro-HPLC peak focusing for the detection and quantification of low hepcidin concentrations.
Publication date :
July 2011
Journal title :
Journal of Separation Science
ISSN :
1615-9306
eISSN :
1615-9314
Publisher :
Wiley-VCH Verlag Gmbh, Weinheim, Germany
Volume :
34
Issue :
15
Pages :
1820-1827
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE] F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
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