Article (Scientific journals)
Effects of marginal iron overload on iron homeostasis and immune function in alveolar macrophages isolated from pregnant and normal rats.
Ward, Roberta J; Wilmet, Stephanie; Legssyer, Rachida et al.
2009In Biometals, 22 (2), p. 211-23
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Keywords :
Animals; Female; Homeostasis; Immune System; Inflammation; Iron/toxicity; Macrophages/cytology/metabolism; NADPH Oxidase/metabolism; NF-kappa B/metabolism; Nitric Oxide Synthase/metabolism; Pregnancy; Pregnancy, Animal; Pulmonary Alveoli/metabolism; Rats; Rats, Wistar
Abstract :
[en] The effects of changes in macrophage iron status, induced by single or multiple iron injections, iron depletion or pregnancy, on both immune function and mRNA expression of genes involved in iron influx and egress have been evaluated. Macrophages isolated from iron deficient rats, or pregnant rats at day 21 of gestation, either supplemented with a single dose of iron dextran, 10 mg, at the commencement of pregnancy, or not, showed significant increases of macrophage ferroportin mRNA expression, which was paralleled by significant decreases in hepatic Hamp mRNA expression. IRP activity in macrophages was not significantly altered by iron status or the inducement of pregnancy +/- a single iron supplement. Macrophage immune function was significantly altered by iron supplementation and pregnancy. Iron supplementation, alone or combined with pregnancy, increased the activities of both NADPH oxidase and nuclear factor kappa B (NFkappaB). In contrast, the imposition of pregnancy reduced the ability of these parameters to respond to an inflammatory stimuli. Increasing iron status, if only marginally, will reduce the ability of macrophages to mount a sustained response to inflammation as well as altering iron homeostatic mechanisms.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Ward, Roberta J
Wilmet, Stephanie
Legssyer, Rachida
Leroy, Danielle ;  Centre Hospitalier Universitaire de Liège - CHU > Service administration des patients
Toussaint, Louise ;  Université de Liège - ULiège > Chimie et bio-industries > Centre de Bio. Fond. - Section de Biologie cell. et moléc.
Crichton, Robert R
Pierreux, Christophe
Hue, Louis
Piette, Jacques ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Virologie - Immunologie - Département des sciences de la vie - GIGA-Research
Srai, Surjit Kaila
Solanky, Nita
Klein, Dominique
Summer, Karl
More authors (3 more) Less
Language :
English
Title :
Effects of marginal iron overload on iron homeostasis and immune function in alveolar macrophages isolated from pregnant and normal rats.
Publication date :
April 2009
Journal title :
Biometals
ISSN :
0966-0844
eISSN :
1572-8773
Publisher :
Kluwer Academic Publishers, Dordrecht, Netherlands
Volume :
22
Issue :
2
Pages :
211-23
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 11 January 2012

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